Biochemical characterization of insecticide resistance in field population of major JE vectors from northern districts of West Bengal, India

2021 ◽  
Author(s):  
Sudeep Ballav ◽  
Moytrey Chatterjee ◽  
Ashif Ali Sardar ◽  
Ushnish Guha ◽  
Subhasish Kamal Guha ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
West Bengal ◽  
Biochemical Characterization ◽  
Field Population

Molecular and biochemical characterization of a sand fly population from Sri Lanka: evidence for insecticide resistance due to altered esterases and insensitive acetylcholinesterase

2005 ◽  
Vol 95 (4) ◽  
pp. 371-380 ◽  
Author(s):  
S.N. Surendran ◽  
S.H.P.P. Karunaratne ◽  
Z. Adamsn ◽  
J. Hemingway ◽  
N.J. Hawkes
Keyword(s):  
Sri Lanka ◽  
Insecticide Resistance ◽  
Biochemical Characterization ◽  
Preliminary Evidence ◽  
Resistance Mechanisms ◽  
Sand Fly ◽  
Cross Resistance ◽  
Cytochrome P450 Monooxygenases ◽  
Sand Flies

AbstractWith an increasing incidence of cutaneous leishmaniasis in Sri Lanka, particularly in northern provinces, insecticide-mediated vector control is under consideration. Optimizing such a strategy requires the characterization of sand fly populations in target areas with regard to species composition and extant resistance, among other parameters. Sand flies were collected by human bait and cattle-baited net traps on Delft Island, used as an illegal transit location by many refugees returning to the north of Sri Lanka from southern India where leishmaniasis is endemic. For species identification, genomic DNA was extracted and a fragment of the ribosomal 18S gene amplified. The sequence from all flies analysed matched that of Phlebotomus argentipes Annandale & Brunetti, the primary vector in India and the most likely vector in Sri Lanka. Independent morphological analysis also identified P. argentipes. To establish the current susceptibility status of vector species, data were obtained at the biochemical level, from which potential cross-resistance to alternative insecticides can be predicted. The Delft Island collection was assayed for the activities of four enzyme systems involved in insecticide resistance (acetylcholinesterase, non-specific carboxylesterases, glutathione-S-transferases and cytochrome p450 monooxygenases), establishing baselines against which subsequent collections can be evaluated. There was preliminary evidence for elevated esterases and altered acetylcholinesterase in this population, the first report of these resistance mechanisms in sand flies to our knowledge, which probably arose from the malathion-based spraying regimes of the Anti-Malarial Campaign.

scholarly journals Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece

2020 ◽  
Author(s):  
Kyriaki Maria Papapostolou ◽  
Maria Riga ◽  
Jason Charamis ◽  
Evangelia Skoufa ◽  
Vassilis Souchlas ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Tetranychus Urticae ◽  
Field Population ◽  
Identification And Characterization
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