Determination of nickel in biological samples prepared by microwave dissolution using electrothermal atomic absorption spectrometry after extraction with 1,5-bis[pheny-(2-pyridyl)methylene]thiocarbonhydrazide

The determination of trace and toxic elements in biological samples (blood, urine and scalp hair samples) of human beings is an important clinical test. The aim of our present study was to determine the concentration of arsenic (As), copper (Cu), cobalt (Co) and manganese (Mn), in biological samples of male production workers (PW) and quality control workers (QW) of steel mill, with aged 25–55 years, to assess the possible influence of environmental exposure. For comparison purpose, the same biological samples of unexposed healthy males of same age group were collected as control subjects. The determination of all elements in biological samples was carried out by electrothermal atomic absorption spectrometry, prior to microwave assisted acid digestion. The accuracy of the As, Cu, Co and Mn measurements was tested by simultaneously analyzing certified reference materials (CRMs) and for comparative purposes conventional wet acid digestion method was used on the same CRMs. No significant differences were observed between the analytical results and the certified values, using both methods (paired t-test at P > 0.05). The results indicate that concentrations of As, Cu, Co and Mn in all three biological samples of the exposed workers (QW and PW) were significantly higher than those of the controls. The possible correlation of these elements with the etiology of different physiological disorders is discussed. The results were also demonstrated the need of attention for improvements in workplace, ventilation and industrial hygiene practices.

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Determination of Cadmium and Lead in Biological Samples by Three Ultrasonic-Based Samples Treatment Procedures Followed by Electrothermal Atomic Absorption Spectrometry

Journal of AOAC International ◽

10.1093/jaoac/90.2.470 ◽

2007 ◽

Vol 90 (2) ◽

pp. 470-478 ◽

Cited By ~ 32

Author(s):

Hassan I Afridi ◽

Tasneem G Kazi ◽

Mohammad B Arain ◽

Mohammad K Jamali ◽

Gul H Kazi ◽

...

Keyword(s):

Atomic Absorption Spectrometry ◽

Atomic Absorption ◽

Biological Samples ◽

Human Hair ◽

Electrothermal Atomic Absorption Spectrometry ◽

Absorption Spectrometry ◽

Bovine Liver ◽

Sample Treatment ◽

Ultrasonic Assisted

Abstract The development of 3 different ultrasonic-based sample treatment methods, ultrasonic probe-assisted acid extraction, ultrasonic-assisted acid slurry, and ultrasonic-assisted acid pseudodigestion is presented. These methods were compared for the determination of Cd and Pb by electrothermal atomic absorption spectrometry in biological samples (blood and scalp hair) and validated by using certified materials BCR 397 human hair and BCR 185R bovine liver. The sample amounts chosen to perform the analysis were 100 mg and 0.5 mL for solids (human hair and bovine liver) and blood samples, respectively. An acid digestion induced by microwave energy was used to obtain the total metal concentrations and for comparative purposes. The best results were obtained with the ultrasonic-assisted acid pseudodigestion, with which it was possible to perform accurate and precise determination of the Cd and Pb contents in 2 certified reference materials and biological samples of 50 normal males of ages 2540 years. The precision of the methods, together with their efficiency, rapidity, low cost, and environmental acceptability, make them good alternatives for the determination of trace metals from biological samples. The precision of the methods for accuracy evaluation, resulting in good agreement according to the t-test for a 95% confidence level, and the relative standard deviations were lower than 10% (n = 10) for all determinations.

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