Determination of protein-bound phosphate by continuous flow analysis: Automated procedure for the determination of alkali-labile phosphate

1983 ◽  
Vol 130 (2) ◽  
pp. 311-320 ◽  
Author(s):  
Kathrin McCollum ◽  
George Taborsky
1969 ◽  
Vol 15 (12) ◽  
pp. 1162-1170 ◽  
Author(s):  
Robert E Wenk ◽  
Ronald J Creno ◽  
Valerie Loock ◽  
John Bernard Henry

Abstract The method described is based on Dubowski’s o-toluidine procedure for the determination of glucose in plasma, serum, or cerebrospinal fluid. It utilizes the rapidity, precision, and closed system of continuous flow analysis of an unmodified AutoAnalyzer to substantially increase sensitivity and accuracy in the low range. The method is more specific than the ferricyanide reduction method. Protein precipitation or dialysis are unnecessary except for hemolyzed specimens and only 0.042 ml of sample are required. The method is inexpensive, easily performed, and compares favorably on a practical basis with enzymatic, reducing, and other aniline dye methods.


2013 ◽  
Vol 47 (21) ◽  
pp. 12325-12332 ◽  
Author(s):  
Helle Astrid Kjær ◽  
Paul Vallelonga ◽  
Anders Svensson ◽  
Magnus Elleskov L. Kristensen ◽  
Catalin Tibuleac ◽  
...  

Author(s):  
T. F. Hartley

Species in urine which were likely to interfere with the determination of ammonia by Nesslerisation were adsorbed onto a precipitate of zinc hydroxide formed in situ in each sample. The ammonia concentrations in the subsequent supernatant solutions were measured using the Nessler method, adapted for use on a continuous flow analysis system. These measurements were made against a series of standards prepared from Analar grade ammonium chloride. The accuracy, reproducibility, and sensitivity of this adaptation were examined and were found to be within acceptable limits.


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