Studies on plasma iron in the rat. II. Plasma iron concentration and plasma iron-binding capacity

Abstract. The experiment was carried out on 14 kids of Polish Improved White breed during the first 30 days of life. The aim of this study was to show changes in the concentration of blood plasma iron and total iron binding capacity (TIBC) during the neonatal period. The statistically confirmed differences (P≥0.01) in blood serum iron concentration was show in the kids between 5th (20.57 μmol/l) and 14th (9.97 μmol/l) day of life, and between 3rd and 4th week with the peak in 23rd day (27.50 μmol/l). We have also shown statisticly confirmed increase in TIBC (P≥0.01) between 1st and 4th day of life (+20.13 μmol/l), and between 14th and 23rd day of life (+15.59 μmol/l). Statistically confirmed decrease of TIBC was observed between 6th (52.30 μmol/l) and 14th (31.78 μmol/l) day of life. Conclusion: The studies have revealed dynamic changes in the concentration and total binding capacity of blood plasma iron concentration during the first month of postnatal life. The pattern of such changes does not depend on the gender or litter size. It was observed a significant decrease in the concentration of this trace element in blood plasma toward the end of the second week, which may indicate a relative iron deficiency, particularly in twin-born kids.

Download Full-text

PLASMA IRON AND IRON-BINDING CAPACITY LEVELS IN HEALTH AND DISEASE : WITH AN IMPROVED METHOD FOR THE ESTIMATION OF PLASMA IRON CONCENTRATION AND TOTAL IRON-BINDING CAPACITY

Australasian Annals of Medicine ◽

10.1111/imj.1960.9.3.209 ◽

1960 ◽

Vol 9 (3) ◽

pp. 209-213 ◽

Cited By ~ 25

Author(s):

E. H. MORGAN ◽

GRAEME CARTER

Keyword(s):

Binding Capacity ◽

Iron Concentration ◽

Total Iron ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Improved Method ◽

Plasma Iron ◽

Health And Disease ◽

Iron Binding Capacity

Download Full-text

PLASMA IRON AND IRON-BINDING CAPACITY

The Lancet ◽

10.1016/s0140-6736(75)92573-8 ◽

1975 ◽

Vol 305 (7919) ◽

pp. 1293

Author(s):

Terry Hamblin

Keyword(s):

Binding Capacity ◽

Iron Binding ◽

Plasma Iron ◽

Iron Binding Capacity

Download Full-text

Iron preparations for children

Drug and Therapeutics Bulletin ◽

10.1136/dtb.4.3.9 ◽

1966 ◽

Vol 4 (3) ◽

pp. 9-11

Keyword(s):

Bone Marrow ◽

Iron Deficiency ◽

Blood Smear ◽

Binding Capacity ◽

Iron Binding ◽

Iron Stores ◽

Plasma Iron ◽

Hypochromic Anaemia ◽

Iron Binding Capacity

We have discussed iron preparations for adults in earlier articles;1 much of the information applies equally to children. Iron is not a ‘tonic’ and should be given only to prevent or correct iron deficiency. Estimation of the haemoglobin and inspection of a blood smear are the minimum investigations necessary before iron is prescribed in therapy. When deficiency is suspected in the absence of hypochromic anaemia, plasma iron and iron-binding capacity should be estimated and/or the bone marrow examined for haemosiderin crystals which disappear when iron stores are depleted.

Download Full-text

Plasma iron and transferrin iron-binding capacity evaluated by colorimetric and immunoprecipitation methods.

Clinical Chemistry ◽

10.1093/clinchem/33.2.273 ◽

1987 ◽

Vol 33 (2) ◽

pp. 273-277 ◽

Cited By ~ 35

Author(s):

H A Huebers ◽

M J Eng ◽

B M Josephson ◽

N Ekpoom ◽

R L Rettmer ◽

...

Keyword(s):

Isotope Dilution ◽

Binding Capacity ◽

Colorimetric Method ◽

Isotope Dilution Method ◽

Dilution Method ◽

Iron Binding ◽

Plasma Iron ◽

Excess Iron ◽

Iron Deficient ◽

Iron Binding Capacity

Abstract We evaluated plasma iron (PI) and total iron-binding capacity (TIBC) or transferrin in normal individuals and in patients with iron imbalance. The standard colorimetric measurements of PI and TIBC and the standard isotope-dilution measurement of TIBC were compared with an immunoprecipitation method and also with immunoelectrophoresis of transferrin. PI concentrations as measured by the standard and immunoprecipitation methods agreed closely for all individuals except those with saturated transferrin, where nontransferrin iron increased the results in the standard assay. This excess iron in saturated plasma may be derived from either free iron or iron-bearing ferritin. There were also differences in TIBC between the two methods. Iron-deficient sera gave higher values for transferrin when measured by immunoelectrophoresis. Unsaturated iron-binding capacity was increased in the isotope-dilution method in some iron-saturated plasma, compounding errors when added to erroneously high PI values to compute TIBC. Perhaps some exchange of iron occurred between added iron and transferrin iron in the isotope-dilution method. These measurements confirm the accuracy of the standard colorimetric method of measuring PI and TIBC except in iron-saturated plasma. However, the greater specificity of a polyclonal immunoprecipitation method of measuring PI and TIBC makes it particularly useful in differentiating transferrin-bound iron from nontransferrin iron.

Download Full-text