The oxidation of long-chain unsaturated fatty acids by isolated rat liver mitochondria as a function of substrate concentration

1978 ◽  
Vol 503 (3) ◽  
pp. 437-449 ◽  
Author(s):  
Willem J. Vaartjes ◽  
Simon G. van den Bergh
Keyword(s):  
Fatty Acids ◽  
Rat Liver ◽  
Substrate Concentration ◽  
Unsaturated Fatty Acids ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Long Chain ◽  
Isolated Rat

scholarly journals A role for 2,4-enoyl-CoA reductase in mitochondrial β-oxidation of polyunsaturated fatty acids. Effects of treatment with clofibrate on oxidation of polyunsaturated acylcarnitines by isolated rat liver mitochondria

1982 ◽  
Vol 208 (3) ◽  
pp. 749-757 ◽  
Author(s):  
H Osmundsen ◽  
J Cervenka ◽  
J Bremer
Keyword(s):  
Fatty Acids ◽  
Rat Liver ◽  
Opposite Effect ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Long Chain Fatty Acids ◽  
Beta Oxidation ◽  
Isolated Rat Liver ◽  
Coa Reductase ◽  
Isolated Rat

1. beta-Oxidation of gamma-linolenoylcarnitine, arachidonoylcarnitine and docosahexaenoylcarnitine by isolated rat liver mitochondria is inhibited by uncoupling conditions. Partial re-activation is obtained with added ATP. With mitochondria from clofibrate-treated rats ATP-stimulated rates of beta-oxidation of docosahexaenoylcarnitine are higher than ADP-stimulated rates. This is not observed with the beta-oxidation of oleoylcarnitine. 2. beta-Oxidation of docosahexaenoylcarnitine, in the presence of rotenone, is inhibited by added oxaloacetate, analogous to previous findings with pent-4-enoylcarnitine [see Osmundsen (1978) FEBS Lett. 88, 219-222]. In the absence of rotenone added oxaloacetate stimulates the beta-oxidation of docosahexaenoylcarnitine, but has the opposite effect on the beta-oxidation of palmitoylcarnitine. 3. beta-Oxidation of polyunsaturated acylcarnitines by isolated rat liver mitochondria is selectively increased after treatment of the animals with a low dietary dose (0.2%, w/w) of clofibrate. Treatment with a higher dose of clofibrate (0.5%, w/w) resulted in a general stimulation of beta-oxidation. 4. The results presented suggest that long-chain fatty acids possessing a delta 4-double bond are not readily beta-oxidized unless the 2,4-enoyl-CoA reductase (EC 1.3.1.-) is operating.

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