Effects of temperature on water diffusion in human erythrocytes and ghosts — nuclear magnetic resonance studies

1987 ◽  
Vol 905 (2) ◽  
pp. 339-348 ◽  
Author(s):  
Gheorghe Benga ◽  
Victor Ioan Pop ◽  
Octavian Popescu ◽  
Adriana Hodârnǎu ◽  
Victoria Borza ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Water Diffusion ◽  
Human Erythrocytes ◽  
Magnetic Resonance Studies ◽  
Effects Of Temperature ◽  
Nuclear Magnetic

Water exchange through erythrocyte membranes: p-choloromercuribenzene sulfonate inhibition of water diffusion in ghosts studied by a nuclear magnetic resonance technique

1985 ◽  
Vol 5 (3) ◽  
pp. 223-228 ◽  
Author(s):  
Gheorghe Benga ◽  
Octavian Popescu ◽  
Victor I. Pop
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Relaxation Time ◽  
Magnetic Resonance ◽  
Water Channel ◽  
Inhibitory Effect ◽  
Water Diffusion ◽  
Human Erythrocytes ◽  
Erythrocyte Membranes ◽  
Diffusional Permeability ◽  
Nuclear Magnetic

A comparison of water diffusion in human erythrocytes and ghosts revealed a longer relaxation time in ghosts, A comparison of water diffusion in human erythrocytes and ghosts revealed a longer relaxation time in ghosts, corresponding to a decreased exchange rate. However, the diffusional permeability of ghosts was not significantly different from that of erythrocytes. The changes in water diffusion following exposure to p-chloromercuribenzene sulfonate (PCMBS) have been studied on ghosts suspended in isotonic solutions. It was found that a significant inhibitory effect of PCMBS on water diffusion occurred only after several minutes of incubation at 37°C. No inhibition was noticed after short incubation at 0°C as previously used in some labelling experiments. This indicates the location in the membrane interior of the SH groups involved in water diffusion across human erythrocyte membranes. The nuclear magnetic resonance (n. m. r.) method appears as a useful tool for studying changes in water diffusiofl in erythrocyte ghosts with the aim of locating the water channel.

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