The role of membrane protein sulfhydryl groups in hydrogen peroxide-mediated membrane damage in human erythrocytes

1988 ◽  
Vol 937 ◽  
pp. 229-240 ◽  
Author(s):  
L.M. Snyder ◽  
N.L. Fortier ◽  
L. Leb ◽  
J. McKenney ◽  
J. Trainor ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Membrane Protein ◽  
Membrane Damage ◽  
Human Erythrocytes ◽  
Sulfhydryl Groups ◽  
Protein Sulfhydryl ◽  
Protein Sulfhydryl Groups

Potassium-chloride cotransport in resealed human red cell ghosts

1986 ◽  
Vol 250 (4) ◽  
pp. C578-C583 ◽  
Author(s):  
P. B. Dunham ◽  
P. J. Logue
Keyword(s):  
Membrane Protein ◽  
Potassium Chloride ◽  
Cell Volume ◽  
Human Erythrocytes ◽  
Sulfhydryl Groups ◽  
Red Cell ◽  
Intact Cells ◽  
Protein Sulfhydryl ◽  
Protein Sulfhydryl Groups ◽  
Resealed Ghosts

Furosemide-inhibitable K influx is threefold higher in resealed ghosts of human erythrocytes than in intact cells. The enhancement is specific for K in that furosemide-inhibitable Na influx is the same in resealed ghosts and intact cells. The enhanced K influx resembles K-Cl cotransport in intact cells in that it requires Cl but not Na. N-ethylmaleimide (NEM), which stimulates furosemide-inhibitable K influx in intact cells, is without effect (or slightly inhibitory) in resealed ghosts. The failure of NEM to enhance the flux was not due to low ATP in the ghosts. These findings suggest that enhancement of the K flux in ghosts occurs by oxidation of membrane protein sulfhydryl groups, known to occur with lysis, the same sulfhydryl groups at which NEM acts by alkylation. This conclusion is supported by two observations: dithiothreitol completely prevents the increase in K influx in ghosts; this agent inhibits both oxidation of sulfhydryl groups and alkylation of them by NEM; and K influx in resealed ghosts is sensitive to changes in cell volume, just as it is in NEM-treated intact cells.

Lipopolysaccharide from Escherichia coli prevents indomethacin-induced gastric damage in rats: role of non-protein sulfhydryl groups and leukocyte adherence

2009 ◽  
Vol 58 (10) ◽  
pp. 717-723 ◽  
Author(s):  
Antoniella Souza Gomes ◽  
Henrique Paula Lemos ◽  
Jand Venes Rolim Medeiros ◽  
Fernando Queiroz Cunha ◽  
Marcellus Henrique Loiola Ponte Souza
Keyword(s):  
Escherichia Coli ◽  
Sulfhydryl Groups ◽  
Gastric Damage ◽  
Leukocyte Adherence ◽  
Protein Sulfhydryl ◽  
Protein Sulfhydryl Groups

Amifostine (Wr-2721) Prevents Indomethacin-Induced Gastric Damage in Rats: Role of Non-Protein Sulfhydryl Groups and Leukocyte Adherence

2006 ◽  
Vol 52 (1) ◽  
pp. 119-125 ◽  
Author(s):  
José Maurício S. C. Mota ◽  
Pedro M. G. Soares ◽  
Álvaro A. J. Menezes ◽  
Henrique P. Lemos ◽  
Fernando Q. Cunha ◽  
...  
Keyword(s):  
Sulfhydryl Groups ◽  
Gastric Damage ◽  
Leukocyte Adherence ◽  
Protein Sulfhydryl ◽  
Protein Sulfhydryl Groups

scholarly journals Gastroprotective effect of the alkaloid boldine: Involvement of non-protein sulfhydryl groups, prostanoids and reduction on oxidative stress

2020 ◽  
Vol 327 ◽  
pp. 109166
Author(s):  
Thaise Boeing ◽  
Luisa Natália Bolda Mariano ◽  
Ana Caroline dos Santos ◽  
Bianca Tolentino ◽  
Angela Cadorin Vargas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sulfhydryl Groups ◽  
Gastroprotective Effect ◽  
Protein Sulfhydryl ◽  
Protein Sulfhydryl Groups
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