The effect of pH on the allosteric properties of baker's yeast cytosine deaminase

1973 ◽  
Vol 315 (2) ◽  
pp. 443-448
Author(s):  
Ettore Balestreri ◽  
Romano Angelo Felicioli ◽  
Pier Luigi Ipata
Biochemistry ◽  
1971 ◽  
Vol 10 (23) ◽  
pp. 4270-4276 ◽  
Author(s):  
Piero L. Ipata ◽  
Franco Marmocchi ◽  
Giulio Magni ◽  
Romano Felicioli ◽  
Giovanni Polidoro

1989 ◽  
Vol 53 (5) ◽  
pp. 1313-1319 ◽  
Author(s):  
Tohoru KATSURAGI ◽  
Toshihiro SONODA ◽  
Kin''ya MATSUMOTO ◽  
Takuo SAKAI ◽  
Kenzo TONOMURA

1952 ◽  
Vol 8 ◽  
pp. 537-542 ◽  
Author(s):  
E.H. Wiggins ◽  
P.F.E. Mann ◽  
W.E. Trevelyan ◽  
J.S. Harrison

1968 ◽  
Vol 109 (3) ◽  
pp. 361-368 ◽  
Author(s):  
C. Cennamo ◽  
G. Montecuccoli ◽  
G. Bonaretti ◽  
L. Razzoli

1. The sensitivity of the NAD+-specific isocitrate dehydrogenase from baker's yeast towards inhibition by anions decreases with decrease in pH. The patterns of the pH-dependence of the enzymic activity can be explained by this effect. 2. In the presence of a high isocitrate concentration, citrate, unlike AMP, has no antagonizing effect on the inhibition of the enzyme by anions. In the presence of AMP, citrate inhibits the enzyme at high isocitrate concentration and activates at low isocitrate concentration. 3. The effects on the enzymic activity of the previous incubation of the enzyme were studied in relation to the substrate concentration, the chloride concentration and the presence of citrate and AMP.


2015 ◽  
Vol 4 (2) ◽  
pp. 27-31 ◽  
Author(s):  
Irvan ◽  
Popphy Prawati ◽  
Bambang Trisakti

Bioethanol is ethanol, which made from starch, glucose, or cellulose of plants. In this research, it has been made from cellulose of sugarcane baggase. This research was conducted to study about the effect of pH, yeast type and the duration of fermentation to yield’s ethanol. The main processes in this research were thermal hydrolysis by liquid hot water (LHW) method, fermentation by using  Saccharomyces cerevisiae and purification by vacuum distillation. The variables in this research were pH 4; 4,5; and 5; baker’s yeast and fermented glutinous cassava; and the duration of fermentation were 2, 4, 6, 8 and 10 days. Based on the analysis of the yield, the volume of bioethanol is 23 mL with 0,95 g/ml density and 161,59 kkal/kg heat value as the best yield, under the condition of  pH 5 and 4 days of the duration of fermentation using baker’s yeast.


2014 ◽  
Vol 13 (12) ◽  
pp. 3153-3160 ◽  
Author(s):  
Zakaria Al-Qodah ◽  
Mohammad Al-Shannag ◽  
Kholoud Alananbeh ◽  
Nahla Bouqellah ◽  
Eman Assirey ◽  
...  

Author(s):  
Ida N Jamal ◽  
Reiny A Tumbol ◽  
Remy E.P Mangindaan

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©


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