Postnatal development of palmitate oxidation and mitochondrial enzyme activities in rat cardiac and skeletal muscle

The adaptation of mitochondrial ATP production rate (MAPR) to training and detraining was evaluated in nine healthy men. Muscle samples (approximately 60 mg) were obtained before and after 6 wk of endurance training and after 3 wk of detraining. MAPR was measured in isolated mitochondria by a bioluminometric method. In addition, the activities of mitochondrial and glycolytic enzymes were determined in skeletal muscle. In response to training, MAPR increased by 70%, with a substrate combination of pyruvate + palmitoyl-L-carnitine + alpha-ketoglutarate + malate, by 50% with only pyruvate + malate, and by 92% with palmitoyl-L-carnitine + malate. With detraining MAPR decreased by 12–28% from the posttraining rate (although not significantly for all substrates). No differences were found when MAPR was related to the protein content in the mitochondrial fraction. The largest increase in mitochondrial enzyme activities induced by training was observed for cytochrome-c oxidase (78%), whereas succinate cytochrome c reductase showed only an 18% increase. The activity of citrate synthase increased by 40% and of glutamate dehydrogenase by 45%. Corresponding changes in maximal O2 uptake were a 9.6% increase by training and a 6.0% reversion after detraining. In conclusion, both MAPR and mitochondrial enzyme activities are shown to increase with endurance training and to decrease with detraining.

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Influence of Local Administration of Serotonin on Mitochondrial Enzyme Activities of Rat Skeletal Muscle and Action of Anti-inflammatory Drugs on It

YAKUGAKU ZASSHI ◽

10.1248/yakushi1947.96.2_132 ◽

1976 ◽

Vol 96 (2) ◽

pp. 132-136

Author(s):

YUICHIRO ARAI ◽

YOSHIO AIZAWA

Keyword(s):

Skeletal Muscle ◽

Enzyme Activities ◽

Local Administration ◽

Mitochondrial Enzyme ◽

Rat Skeletal Muscle ◽

Inflammatory Drugs ◽

Anti Inflammatory

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Effects of Chronic Ethanol Abuse on Structure and Enzyme Activities of Skeletal Muscle in Man

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.3109/00365517509095786 ◽

1975 ◽

Vol 35 (6) ◽

pp. 601-607 ◽

Cited By ~ 37

Author(s):

K. -H. Kiessling ◽

L. Pilström ◽

A. -C. Bylund ◽

K. Piehl ◽

B. Saltin

Keyword(s):

Skeletal Muscle ◽

Enzyme Activities ◽

Chronic Ethanol ◽

Ethanol Abuse

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INFLUENCE OF LOCAL ADMINISTRATION OF SEROTONIN ON MITOCHONDRIAL ENZYME ACTIVITY OF RAT SKELETAL MUSCLE

The Japanese Journal of Pharmacology ◽

10.1254/jjp.26.261 ◽

1976 ◽

Vol 26 (2) ◽

pp. 261-263

Author(s):

Yuichiro ARAI ◽

Yoshio AIZAWA

Keyword(s):

Skeletal Muscle ◽

Enzyme Activity ◽

Local Administration ◽

Mitochondrial Enzyme ◽

Rat Skeletal Muscle

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Enzyme studies of skeletal muscle in mice with hereditary muscular dystrophy

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1963.205.5.897 ◽

1963 ◽

Vol 205 (5) ◽

pp. 897-901 ◽

Cited By ~ 45

Author(s):

Marilyn W. McCaman

Keyword(s):

Skeletal Muscle ◽

Muscular Dystrophy ◽

Glutathione Reductase ◽

Enzyme Activities ◽

Lactic Dehydrogenase ◽

Normal Muscle ◽

Dystrophic Muscle ◽

Nerve Section ◽

Mouse Muscle ◽

Dystrophic Mouse

The activities of 20 enzymes in normal, heterozygous, and dystrophic mouse muscle were studied by means of quantitative microchemical methods. Enzyme activities in normal and heterozygous muscle were essentially the same. In dystrophic muscle glucose-6-P dehydrogenase, 6-P-gluconic dehydrogenase, glutathione reductase, peptidase, ß-glucuronidase, and glucokinase activities were significantly higher than in normal muscle, while α-glycero-P dehydrogenase and lactic dehydrogenase activities were significantly lower. The pattern of enzyme activities found in normal gastrocnemius denervated by nerve section was strikingly similar to that in dystrophic muscle.

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