The quantitative determination of metabolites of 6-mercaptopurine in biological materials III. The determination of 14C-labeled 6-thiopurines in L5178Y cell extracts using high-pressure liquid cation-exchange chromatography

1978 ◽  
Vol 518 (2) ◽  
pp. 205-215 ◽  
Author(s):  
Hans-Joachim Breter ◽  
Armin Maidhof ◽  
Rudolf K. Zahn
Keyword(s):  
High Pressure ◽  
Quantitative Determination ◽  
Cation Exchange ◽  
Biological Materials ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Cell Extracts ◽  
L5178y Cell

A method for determination of macropeptide by cation-exchange fast protein liquid chromatography and its use for following the action of chymosin in milk

1991 ◽  
Vol 58 (3) ◽  
pp. 321-328 ◽  
Author(s):  
Joëlle Léonil ◽  
Daniel Mollé
Keyword(s):  
Quantitative Determination ◽  
Cation Exchange ◽  
Soluble Fraction ◽  
Whey Proteins ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Chromatographic Technique ◽  
Cation Exchange Chromatography ◽  
Preparative Scale

SummaryCation-exchange chromatography on a Mono S column (Pharmacia) was used to separate macropeptide from whey proteins. Macropeptide was eluted by 0·1 M-NaCl in a 20 mM-KCl–HCl buffer, pH 2. This technique was suitable for quantitative determination of macropeptide in rennet whey and also for following the action of chymosin on κ-casein in skim milk. Precipitation at pH 4·6 was used to remove residual caseins and to keep macropeptide in solution. In comparison with other methods for determining macropeptide, the present one eliminates the need for pretreatment of samples with trichloroacetic acid (TCA) and allows the recovery of all the macropeptide. Quantitative determination of macropeptide in the 8% TCA-soluble fraction by cation-exchange chromatography showed that only 50–75% of the macropeptide was recovered. This chromatographic technique could also be applied for isolating and producing whole macropeptide on a preparative scale.

The Determination of the DNA Base Composition in 19 Species of Adriatic Sponges with High-Pressure Liquid Cation-Exchange Chromatography

1976 ◽  
Vol 31 (9-10) ◽  
pp. 554-557 ◽  
Author(s):  
Hans-Joachim Breter ◽  
Ferdinand Hundt ◽  
Rudolf K. Zahn
Keyword(s):  
High Pressure ◽  
Cation Exchange ◽  
Base Composition ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Dna Base Composition ◽  
Dna Base ◽  
Semiquantitative Method ◽  
Adenine Thymine

Abstract The (adenine + thymine)/(guanine + cytosine) base ratios of 19 species of adriatic sponges have been determined by high-pressure liquid cation-exchange chromatography. The base ratios vary from 1.49 (Mycale massa) to 0.63 (Hippospongia communis) according to an (A+T) content of 59.7 and 38.6 mol%, respectively. The DNAs of sponges of the order Keratosa showed marked differences in their (A +T) contents (39.5 to 58.8 mol%) whereas those of Tetractinellida and Halichondrina were nearly identical (39.3 to 40.8 and 49.5 to 49.8 mol%, respectively). The 5-methylcytosine (5MC) content was determined in 8 sponge DNAs by a semiquantitative method. The values differed from 0.8 to 2.2 mol% of 5MC.

Inductively coupled plasma mass spectrometry coupled to cation exchange chromatography for the determination of trace nickel in alkaline electrolyte

2020 ◽  
Vol 35 (7) ◽  
pp. 1295-1299
Author(s):  
Lukas Miner ◽  
Diane Beauchemin
Keyword(s):  
Mass Spectrometry ◽  
Cation Exchange ◽  
Inductively Coupled Plasma ◽  
Exchange Chromatography ◽  
Alkaline Solutions ◽  
Alkaline Electrolyte ◽  
Cation Exchange Chromatography ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry

Cone corrosion by alkaline solutions with flow injection is prevented using cation-exchange chromatography coupled to inductively coupled plasma mass spectrometry.

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