The use of high-pressure liquid cation-exchange chromatography for determination of the 5-methylcytosine content of DNA

1976 ◽  
Vol 118 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Hans-Joachim Breter ◽  
Gerhard Seibert ◽  
Rudolf K. Zahn
Keyword(s):  
High Pressure ◽  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography

The Determination of the DNA Base Composition in 19 Species of Adriatic Sponges with High-Pressure Liquid Cation-Exchange Chromatography

1976 ◽  
Vol 31 (9-10) ◽  
pp. 554-557 ◽  
Author(s):  
Hans-Joachim Breter ◽  
Ferdinand Hundt ◽  
Rudolf K. Zahn
Keyword(s):  
High Pressure ◽  
Cation Exchange ◽  
Base Composition ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Dna Base Composition ◽  
Dna Base ◽  
Semiquantitative Method ◽  
Adenine Thymine

Abstract The (adenine + thymine)/(guanine + cytosine) base ratios of 19 species of adriatic sponges have been determined by high-pressure liquid cation-exchange chromatography. The base ratios vary from 1.49 (Mycale massa) to 0.63 (Hippospongia communis) according to an (A+T) content of 59.7 and 38.6 mol%, respectively. The DNAs of sponges of the order Keratosa showed marked differences in their (A +T) contents (39.5 to 58.8 mol%) whereas those of Tetractinellida and Halichondrina were nearly identical (39.3 to 40.8 and 49.5 to 49.8 mol%, respectively). The 5-methylcytosine (5MC) content was determined in 8 sponge DNAs by a semiquantitative method. The values differed from 0.8 to 2.2 mol% of 5MC.

Inductively coupled plasma mass spectrometry coupled to cation exchange chromatography for the determination of trace nickel in alkaline electrolyte

2020 ◽  
Vol 35 (7) ◽  
pp. 1295-1299
Author(s):  
Lukas Miner ◽  
Diane Beauchemin
Keyword(s):  
Mass Spectrometry ◽  
Cation Exchange ◽  
Inductively Coupled Plasma ◽  
Exchange Chromatography ◽  
Alkaline Solutions ◽  
Alkaline Electrolyte ◽  
Cation Exchange Chromatography ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry

Cone corrosion by alkaline solutions with flow injection is prevented using cation-exchange chromatography coupled to inductively coupled plasma mass spectrometry.

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