Determination of radioactivity-labelled derivatives of [8-14C]-6-mercaptopurine in homogenates of L5178Y mouse lymphoma cells using high-pressure liquid cation-exchange chromatography

1976 ◽  
Vol 279 (2) ◽  
pp. 151-152 ◽  
Author(s):  
H. J. Breter ◽  
R. K. Zahn

1968 ◽  
Vol 14 (5) ◽  
pp. 383-390 ◽  
Author(s):  
Charles D Guri ◽  
Leonard J Cole

Abstract Urinary deoxycytidine and other pyrimidines have been studied in rats after wholebody irradiation. Utilizing combined column and paper chromatography and colorimetric determination of the isolated deoxycytidine, significant elevations in the excretion of this compound were observed during the first 24 hr. after irradiation. An increased concentration of deoxycytidine in the plasma was also observed within 4 hr. after X-irradiation. Utilizing both anion- and cation-exchange chromatography, X-irradiation has been shown to produce an increase in several other pyrimidines, including derivatives of the bases cytosine, thymine, and uracil. While the data were suggestive of a radiation-induced generalized pyrimidinuria, urinary deoxycytidine and thymidine demonstrated remarkable elevation and were predominantly responsible for this phenomenon.





1976 ◽  
Vol 31 (9-10) ◽  
pp. 554-557 ◽  
Author(s):  
Hans-Joachim Breter ◽  
Ferdinand Hundt ◽  
Rudolf K. Zahn

Abstract The (adenine + thymine)/(guanine + cytosine) base ratios of 19 species of adriatic sponges have been determined by high-pressure liquid cation-exchange chromatography. The base ratios vary from 1.49 (Mycale massa) to 0.63 (Hippospongia communis) according to an (A+T) content of 59.7 and 38.6 mol%, respectively. The DNAs of sponges of the order Keratosa showed marked differences in their (A +T) contents (39.5 to 58.8 mol%) whereas those of Tetractinellida and Halichondrina were nearly identical (39.3 to 40.8 and 49.5 to 49.8 mol%, respectively). The 5-methylcytosine (5MC) content was determined in 8 sponge DNAs by a semiquantitative method. The values differed from 0.8 to 2.2 mol% of 5MC.



2020 ◽  
Vol 35 (7) ◽  
pp. 1295-1299
Author(s):  
Lukas Miner ◽  
Diane Beauchemin

Cone corrosion by alkaline solutions with flow injection is prevented using cation-exchange chromatography coupled to inductively coupled plasma mass spectrometry.



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