Tetranuclear and binuclear iron-sulfur clusters in succinate dehydrogenase: A method of iron quantitation by formation of paramagnetic complexes

1976 ◽  
Vol 73 (3) ◽  
pp. 833-840 ◽  
Author(s):  
John C. Salerno ◽  
Tomoko Ohnishi ◽  
Joyce Lim ◽  
Tsoo E. King
Keyword(s):  
Succinate Dehydrogenase ◽  
Iron Sulfur Clusters ◽  
Iron Sulfur ◽  
Paramagnetic Complexes ◽  
Binuclear Iron

The Iron-Sulfur Clusters in Succinate Dehydrogenase

1987 ◽  
pp. 473-484 ◽  
Author(s):  
Michael K. Johnson ◽  
Joyce E. Morningstar ◽  
Edna B. Kearney ◽  
Gary Cecchini ◽  
Brian A. C. Ackrell
Keyword(s):  
Succinate Dehydrogenase ◽  
Iron Sulfur Clusters ◽  
Iron Sulfur

scholarly journals Fe-S cofactors in the SARS-CoV-2 RNA-dependent RNA polymerase are potential antiviral targets

Science ◽  
2021 ◽  
pp. eabi5224
Author(s):  
Nunziata Maio ◽  
Bernard A. P. Lafont ◽  
Debangsu Sil ◽  
Yan Li ◽  
J. Martin Bollinger ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Metal Binding ◽  
Rna Dependent Rna Polymerase ◽  
Iron Sulfur Clusters ◽  
Metal Binding Sites ◽  
Iron Sulfur ◽  
Cryo Electron Microscopy ◽  
Metal Cofactors ◽  
Antiviral Targets ◽  
Viral Helicase

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causal agent of coronavirus disease 2019 (COVID-19), uses an RNA-dependent RNA polymerase (RdRp) for the replication of its genome and the transcription of its genes. We found that the catalytic subunit of the RdRp, nsp12, ligates two iron-sulfur metal cofactors in sites that were modeled as zinc centers in the available cryo-electron microscopy structures of the RdRp complex. These metal binding sites are essential for replication and for interaction with the viral helicase. Oxidation of the clusters by the stable nitroxide TEMPOL caused their disassembly, potently inhibited the RdRp, and blocked SARS-CoV-2 replication in cell culture. These iron-sulfur clusters thus serve as cofactors for the SARS-CoV-2 RdRp and are targets for therapy of COVID-19.

EPR-derived structures of flavin radical and iron-sulfur clusters from Methylosinus sporium 5 reductase

2021 ◽  
Author(s):  
Han Sol Jeong ◽  
Sugyeong Hong ◽  
Hee Seon Yoo ◽  
Jin Kim ◽  
Yujeong Kim ◽  
...  
Keyword(s):  
Carbon Cycle ◽  
Crucial Role ◽  
Methane Monooxygenase ◽  
Global Carbon Cycle ◽  
Ambient Conditions ◽  
Iron Sulfur Clusters ◽  
Iron Sulfur ◽  
Greenhouse Effects ◽  
Global Carbon ◽  
Significant Attention

Methane monooxygenase (MMO) has attracted significant attention owing to its crucial role in the global carbon cycle; it impedes greenhouse effects by converting methane to methanol under ambient conditions. The...

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