The effects of growth hormone-releasing hormone (GH-RH) and thyroxine on the synthesis and release of GH in a clonal strain of rat pituitary tumor cells

1976 ◽  
Vol 70 (1) ◽  
pp. 241-247 ◽  
Author(s):  
Hiroshi Ishikawa ◽  
Takahisa Nagayama ◽  
Chieko Kato ◽  
Masuo Takahashi
Keyword(s):  
Growth Hormone ◽  
Tumor Cells ◽  
Pituitary Tumor ◽  
Growth Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Clonal Strain ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells

-Aspartate in a Prolactin-Secreting Clonal Strain of Rat Pituitary Tumor Cells (GH3)

2000 ◽  
Vol 276 (3) ◽  
pp. 1143-1147 ◽  
Author(s):  
Zhiqung Long ◽  
Jen-Ai Lee ◽  
Taizo Okamoto ◽  
Noriyuki Nimura ◽  
Kazuhiro Imai ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Pituitary Tumor ◽  
Clonal Strain ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells

scholarly journals Prolactin storage in a clonal strain of rat pituitary tumor cells is cell-cycle dependent.

1982 ◽  
Vol 93 (2) ◽  
pp. 459-462 ◽  
Author(s):  
D R Kiino ◽  
D E Burger ◽  
P S Dannies
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Tumor Cells ◽  
Pituitary Tumor ◽  
Fluorescein Isothiocyanate ◽  
Cell Cycle Distribution ◽  
Clonal Strain ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells ◽  
Cycle Dependent

GH4C1 cells (CH cells) are a clonal strain of rat pituitary tumor cells which secrete prolactin. We measured intracellular prolactin at different stages of the cell cycle using flow microfluorometry. Prolactin was stained by an indirect immunocytochemical technique using fluorescein isothiocyanate (FITC)-conjugated antiserum, and DNA was stained simultaneously with propidium iodide. We found that prolactin storage in GH cells was cell-cycle dependent; prolactin storage increased as cells passed from G1 to S to G2 + M. We have shown previously that insulin and 17 beta-estradiol act synergistically to increase intracellular prolactin three- to sevenfold and slow the rate of cell growth to approximately 70% of control cells. In this study we observed that insulin and estradiol increased prolactin storage at each stage of the cell cycle but did not affect the cell-cycle distribution of the population even though cell growth was slowed. We conclude that insulin and estradiol did not increase prolactin storage by affecting the cell-cycle distribution of the population.

Effect of insulin on growth hormone (GH) induction by cortisol in rat pituitary tumor cells

Life Sciences ◽  
1970 ◽  
Vol 9 (14) ◽  
pp. 805-813 ◽  
Author(s):  
Tapani Vanha-Perttula ◽  
Peter O. Kohler ◽  
Lawrence A. Frohman
Keyword(s):  
Growth Hormone ◽  
Tumor Cells ◽  
Pituitary Tumor ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells
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