The α-bungarotoxin site and its relation to the cholinergic and nerve growth factor mediated increases in tyrosine hydroxylase activity in cultures of sympathetic ganglia and chromaffin cells

1982 ◽  
Vol 244 (2) ◽  
pp. 331-336 ◽  
Author(s):  
M. Quik ◽  
J.M. Trifaró
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Tyrosine Hydroxylase ◽  
Chromaffin Cells ◽  
Sympathetic Ganglia ◽  
Hydroxylase Activity ◽  
Nerve Growth ◽  
Tyrosine Hydroxylase Activity

Antibodies against mouse nerve growth factor interfere in vivo with the development of avian sensory and sympathetic neurones

Development ◽  
1988 ◽  
Vol 103 (3) ◽  
pp. 545-552 ◽  
Author(s):  
H. Rohrer ◽  
M. Hofer ◽  
R. Hellweg ◽  
S. Korsching ◽  
A.D. Stehle ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Tyrosine Hydroxylase ◽  
Marker Enzyme ◽  
Hydroxylase Activity ◽  
Nerve Growth ◽  
Protein Levels ◽  
Sympathetic Neurones

The monoclonal antibody 27/21 directed against mouse nerve growth factor (NGF) interferes in vivo with the survival of sensory dorsal root ganglion (DRG) neurones during the development of the quail embryo: the number of DRG neurones at embryonic day 11 (E11) was reduced by about 30% in embryos treated with the antibody between E3 and E11. Neurone numbers in the nodose ganglion were not affected. The effect of NGF antibodies on sympathetic neurones was assessed by determining the levels of the adrenergic marker enzyme tyrosine hydroxylase. Both total tyrosine hydroxylase activity and protein levels in sympathetic chains were reduced by about 30% in embryos treated with 27/21 antibody but not in embryos treated with a control antibody. The 27/21 antibody cross-reacts with chick NGF-like activity as shown in vitro by the ability of the antibody to partially block the survival activity of chick-embryo-fibroblast-conditioned medium for E9 chick DRG neurones.

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