The role of DNA polymerases in DNA repair in Escherichia coli: DNA polymerase I-dependent repair following chemical alkylation of permeabilized bacteria

1977 ◽  
Vol 18 (3) ◽  
pp. 365-380 ◽  
Author(s):  
Daniel Billen ◽  
G.R. Hellermann
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Dna Polymerase I ◽  
Polymerase I

THE INFLUENCE OF THE PO1A1 MUTATION UPON RECOMBINATION IN ESCHERICHIA COLI K12

1979 ◽  
Vol 21 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Barry W. Glickman ◽  
Tineke Rutgers
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Genetic Recombination ◽  
Dna Polymerase I ◽  
Multiple Gene ◽  
E Coli ◽  
Recombinational Process ◽  
K 12 ◽  
Polymerase I

Genetic recombination in Escherichia coli is a highly regulated process involving multiple gene products. We have investigated the role of DNA polymerase I in this process by studying the effect of the po1A1 mutation upon DNA transfer and conjugation in otherwise isogenic suppressor-free strains of E. coli K-12. It was found that the po1A1 mutation greatly reduces recombination in Hfr crosses (a factor of 20 in Po1+ × Po1A1 crosses and more than a factor of 100 in Po1A1 × Po1A1 crosses). However, since the po1A1 mutation reduces the strains capacity to act as a recipient for an F-prime and the analysis of recombination transfer gradients revealed no differences between Po1+ and Po1− strains, it is concluded that DNA polymerase I probably affects the transfer and/or stability of donor DNA rather than the recombinational process itself.

scholarly journals Role of High-Fidelity Escherichia coli DNA Polymerase I in Replication Bypass of a Deoxyadenosine DNA-Peptide Cross-Link

2011 ◽  
Vol 193 (15) ◽  
pp. 3815-3821 ◽  
Author(s):  
K. Yamanaka ◽  
I. G. Minko ◽  
S. E. Finkel ◽  
M. F. Goodman ◽  
R. S. Lloyd
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
High Fidelity ◽  
Cross Link ◽  
Dna Polymerase I ◽  
Polymerase I

Role of the 5´ → 3´ exonuclease and Klenow fragment of Escherichia coli DNA polymerase I in base mismatch repair

2007 ◽  
Vol 278 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Masaru Imai ◽  
Yu-ichiro Tago ◽  
Makoto Ihara ◽  
Masakado Kawata ◽  
Kazuo Yamamoto
Keyword(s):  
Escherichia Coli ◽  
Mismatch Repair ◽  
Dna Polymerase ◽  
Klenow Fragment ◽  
Dna Polymerase I ◽  
Base Mismatch ◽  
Polymerase I

scholarly journals Rapid Identification of Genes Encoding DNA Polymerases by Function-Based Screening of Metagenomic Libraries Derived from Glacial Ice

2009 ◽  
Vol 75 (9) ◽  
pp. 2964-2968 ◽  
Author(s):  
Carola Simon ◽  
Judith Herath ◽  
Stephanie Rockstroh ◽  
Rolf Daniel
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Rapid Identification ◽  
Dna Polymerase I ◽  
Glacial Ice ◽  
Metagenomic Libraries ◽  
Genes Encoding ◽  
Polymerase I ◽  
Encoding Genes

ABSTRACT Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered.

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