Determination of dehydroepiandrosterone sulfate in human plasma by competitive protein binding

1971 ◽  
Vol 34 (1) ◽  
pp. 77-83 ◽  
Author(s):  
R. Hampl ◽  
L. Stárka
Keyword(s):  
Protein Binding ◽  
Human Plasma ◽  
Dehydroepiandrosterone Sulfate ◽  
Competitive Protein Binding

scholarly journals Plasma Cortisone in Man: Its Determination, Physiological Variation, and Significance

1980 ◽  
Vol 17 (5) ◽  
pp. 227-232 ◽  
Author(s):  
J D Few ◽  
G C Cashmore
Keyword(s):  
Protein Binding ◽  
Simultaneous Determination ◽  
Cortisol Level ◽  
Paper Chromatography ◽  
Plasma Proteins ◽  
Hepatic Uptake ◽  
Physiological Variation ◽  
Competitive Protein Binding ◽  
Response To Exercise

In a method for the simultaneous determination of cortisone, cortisol, and corticosterone, the extracted steroids are separated using paper chromatography and assayed by competitive protein binding. Evidence of the reliability of the method, particularly with respect to the determination of cortisone, is presented. Only trivial changes in cortisone level were detected in response to exercise, surgery, and ACTH, even though marked changes in cortisol level were simultaneously recorded. It is suggested that the inertia in plasma cortisone level may be related to its lesser degree of binding to plasma proteins, relative to cortisol, which would permit more rapid hepatic uptake and possible accumulation of a large extravascular pool of cortisone.

Determination of serum testosterone and androstanediol by competitive protein binding

Steroids ◽  
1973 ◽  
Vol 21 (1) ◽  
pp. 27-46 ◽  
Author(s):  
A.L. Strickland ◽  
M. Apland ◽  
J. Bruton
Keyword(s):  
Protein Binding ◽  
Serum Testosterone ◽  
Competitive Protein Binding
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