Development and validation of a highly sensitive LC–MS/MS method for the determination of acacetin in human plasma and its application to a protein binding study

2015 ◽  
Vol 39 (2) ◽  
pp. 213-220 ◽  
Author(s):  
Sang-Bum Kim ◽  
Taehun Lee ◽  
Hun Seok Lee ◽  
Chung Kil Song ◽  
Hyun-Jong Cho ◽  
...  
Keyword(s):  
Protein Binding ◽  
Human Plasma ◽  
Binding Study ◽  
Highly Sensitive ◽  
Development And Validation

Development and validation of a highly sensitive second derivative synchronous fluorescence spectroscopic method for the simultaneous determination of elbasvir and grazoprevir in pharmaceutical preparation and human plasma

2020 ◽  
Vol 44 (43) ◽  
pp. 18679-18685
Author(s):  
Khalid A. M. Attia ◽  
Ahmed El-Olemy ◽  
Sherif Ramzy ◽  
Ahmed H. Abdelazim ◽  
Mohamed A. Hasan ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Pharmaceutical Preparation ◽  
Spectroscopic Method ◽  
Second Derivative ◽  
Highly Sensitive ◽  
Development And Validation

Elbasvir and grazoprevir combination has been newly approved for the treatment of patients infected with hepatitis C virus.

Development and validation of a highly sensitive method for the determination of abiraterone in rat and human plasma by LC-MS/MS-ESI: application to a pharmacokinetic study

2011 ◽  
Vol 26 (6) ◽  
pp. 761-768 ◽  
Author(s):  
Sandip Gurav ◽  
Ravindra Punde ◽  
Junaid Farooqui ◽  
Mohd Zainuddin ◽  
Sriram Rajagopal ◽  
...  
Keyword(s):  
Human Plasma ◽  
Pharmacokinetic Study ◽  
Highly Sensitive ◽  
Development And Validation ◽  
Sensitive Method

scholarly journals METHOD DEVELOPMENT AND VALIDATION OF SELECTIVE AND HIGHLY SENSITIVE METHOD FOR DETERMINATION OF APIXABAN IN HUMAN PLASMA USING LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

2019 ◽  
pp. 39-45
Author(s):  
CHAITANYA KRISHNA ATMAKURI ◽  
CHEEPURUPALLI PRASAD ◽  
RAJARAM S. PATIL ◽  
KARUNAKRANTH DHARANI
Keyword(s):  
Human Plasma ◽  
Method Development ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Highly Sensitive ◽  
Liquid Chromatography Tandem Mass ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Sensitive Method

Objective: The present research work aims to develop and validate a selective and highly sensitive method for the determination of apixaban in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods: 200 µl of sodium heparin plasma samples were acidified and clean-up was performed by using solid-phase extraction (SPE). Apixaban 13C D3 was used as an internal standard (deuterated) to lower the relative matrix effects and a single step SPE was employed for sample clean up. 10 µl of SPE eluent was loaded onto Hypersil Beta Basic C18, 100×4.6 mm, 5 µ column for highly selective chromatographic separation using an isocratic mobile phase. 2 mmol ammonium acetate in water and acetonitrile were delivered by using a quaternary low-pressure gradient pump without premixing at a minimum flow rate of 0.50 ml/min. Results: LC-MS/MS method was successfully developed and validated to demonstrate the lowest detection limit of 0.05 ng/ml and a linear dynamic range from 1-250 ng/ml with r2>0.99. Method development and validation results proved that the method is selective and highly sensitive for the determination of apixaban in human plasma using LC-MS/MS. Conclusion: Current method can be applied for both therapeutic drug monitoring (TDM) and pharmacokinetic (PK) study analysis.

scholarly journals LC–MS/MS assay for the determination of natamycin in rabbit and human plasma: Application to a pharmacokinetics and protein binding study

2013 ◽  
Vol 3 (2) ◽  
pp. 144-148 ◽  
Author(s):  
Yashpal Singh Chhonker ◽  
Devendra Kumar ◽  
Pankaj Shrivastava ◽  
Deepak Kumar ◽  
Rajbir Singh ◽  
...  
Keyword(s):  
Protein Binding ◽  
Human Plasma ◽  
Binding Study

Development and Validation of LC–MS Method for the Determination of Hydroxyzine Hydrochloride in Human Plasma and Subsequent Application in a Bioequivalence Study

2007 ◽  
Vol 66 (7-8) ◽  
pp. 481-486 ◽  
Author(s):  
Neng Zhou ◽  
Yi-Zeng Liang ◽  
Ben-Mei Chen ◽  
Ping Wang ◽  
Xian Chen ◽  
...  
Keyword(s):  
Human Plasma ◽  
Bioequivalence Study ◽  
Subsequent Application ◽  
Development And Validation

Highly sensitive determination of alendronate in human plasma and dialysate using metal-free HPLC-MS/MS

2018 ◽  
Vol 30 ◽  
pp. 14-20 ◽  
Author(s):  
Miho Yamada ◽  
Xiao-Pen Lee ◽  
Masaya Fujishiro ◽  
Ken Iseri ◽  
Makoto Watanabe ◽  
...  
Keyword(s):  
Human Plasma ◽  
Metal Free ◽  
Highly Sensitive ◽  
Sensitive Determination
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