Sensitive assay of creatine kinase isoenzymes in human serum using M subunit inhibiting antibody and firefly luciferase

1978 ◽  
Vol 87 (2) ◽  
pp. 199-209 ◽  
Author(s):  
Arne Lundin ◽  
Inger Styrélius
Keyword(s):  
Creatine Kinase ◽  
Human Serum ◽  
Firefly Luciferase ◽  
Sensitive Assay ◽  
Creatine Kinase Isoenzymes

Radioimmunoassay of creatine kinase isoenzymes in human serum: isoenzyme BB.

1978 ◽  
Vol 24 (3) ◽  
pp. 422-428 ◽  
Author(s):  
M H Zweig ◽  
A C Van Steirteghem ◽  
A N Schechter
Keyword(s):  
Creatine Kinase ◽  
Human Serum ◽  
Cross Reactivity ◽  
Healthy Adults ◽  
Serum Samples ◽  
Creatine Kinase Isoenzymes ◽  
Specific Radioimmunoassay ◽  
Assay Precision ◽  
Free Antigen ◽  
Healthy Persons

Abstract We describe a sensitive, specific radioimmunoassay for the BB isoenzyme of creatine kinase (CK-BB) in serum. A sequential saturation assay was used to achieve sufficient sensitivity to detect the isoenzyme in 100-microliter serum samples of all healthy persons and patients tested. Bound and free antigen were separated by a second antibody system. Large excesses of purified isoenzyme MM did not react in the assay. Cross reactivity of two preparations of CK-MB was only 1 to 7+. The 95th percentile of serum CK-BB in 208 healthy adults was 6.2 microgram/liter. Within-assay and between-assay precision ranged from 5.5 to 11.9% and 9.7 to 13.6%, respectively.

Human creatine kinase: Isoenzymes and logistics of energy distribution

1984 ◽  
Vol 44 (7) ◽  
pp. 611-615 ◽  
Author(s):  
Christer Sylvén ◽  
Eva Jansson ◽  
Anders Kallner ◽  
Kim Böök
Keyword(s):  
Creatine Kinase ◽  
Energy Distribution ◽  
Creatine Kinase Isoenzymes

Creatine kinase isoenzymes in cerebrospinal fluid in a case of brain damage.

1976 ◽  
Vol 22 (8) ◽  
pp. 1405-1407 ◽  
Author(s):  
P M Bayer ◽  
F Gabl ◽  
G Granditsch ◽  
K Widhalm ◽  
H Zyman ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Creatine Kinase ◽  
High Activity ◽  
Brain Damage ◽  
Acute Phase ◽  
Clinical Importance ◽  
Total Activity ◽  
Spinal Fluid ◽  
Consumption Coagulopathy ◽  
Creatine Kinase Isoenzymes

Abstract We present a case of a 11/2-year-old boy with toxic enteritis, consecutive consumption coagulopathy, and sever brain damage. During the acute phase we found high activity of the BB isoenzyme of creatine kinase in cerebrospinal fluid, but not in the serum. Isoenzyme MM could also be found in the spinal fluid (37.9% of the total activity). We conclude that analysis for creatine kinase isoenzymes in spinal fluid is of clinical importance.

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