Stereospecific binding sites for [3H]nicotinamide adenine dinucleotide in the rat brain

1985 ◽  
Vol 116 (1-2) ◽  
pp. 121-127 ◽  
Author(s):  
Penelope H. Snell ◽  
Christopher R. Snell ◽  
Christopher D. Richards
Keyword(s):  
Rat Brain ◽  
Nicotinamide Adenine Dinucleotide ◽  
Binding Sites ◽  
Nicotinamide Adenine

NICOTINAMIDE ADENINE DINUCLEOTIDE LEVELS IN RAT BRAIN

1964 ◽  
Vol 42 (4) ◽  
pp. 489-492 ◽  
Author(s):  
F. Christine Brown
Keyword(s):  
Rat Brain ◽  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine ◽  
Albino Rats ◽  
Deficiency Symptoms ◽  
Severe Deficiency

Albino rats were fed diets which were deficient in tryptophan, niacin, or both, until severe deficiency symptoms appeared. The brains were analyzed for their content of oxidized and reduced nicotinamide adenine dinucleotides (NAD), which are derived from dietary tryptophan and niacin. Deficiencies of tryptophan or niacin had little or no effects on the levels of NAD in rat brain. The injection of niacinamide in massive doses caused a 50 to 75% increase in the NAD levels. Reserpine had no significant effect on brain NAD. No "tranquilizing" or depressing effects were noticed after niacinamide injection.

scholarly journals Release, neuronal effects and removal of extracellular β-nicotinamide adenine dinucleotide (β-NAD+) in the rat brain

2012 ◽  
Vol 35 (3) ◽  
pp. 423-435 ◽  
Author(s):  
Leonie Durnin ◽  
Yanping Dai ◽  
Isamu Aiba ◽  
C. William Shuttleworth ◽  
Ilia A. Yamboliev ◽  
...  
Keyword(s):  
Rat Brain ◽  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine

scholarly journals LOCALIZATION OF NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE-DEPENDENT DEHYDROGENASES IN CATECHOLAMINE-CONTAINING NEURONS OF RAT BRAIN STUDIES ON THE NUCLEUS LOCUS CERULEUS

1974 ◽  
Vol 22 (1) ◽  
pp. 20-28 ◽  
Author(s):  
F. C. KAUFFMAN ◽  
F. E. BLOOM ◽  
K. L. SIMS ◽  
V. M. PICKEL
Keyword(s):  
Brain Stem ◽  
Rat Brain ◽  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Locus Ceruleus ◽  
Nicotinamide Adenine ◽  
High Concentration ◽  
Intense Staining ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose 6 Phosphate Dehydrogenase

Histochemical and cytochemical methods have been used to localize glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, isocitrate (nicotinamide adenine dinucleotide phosphate (NADP)) dehydrogenase and malic enzyme in the nucleus locus ceruleus and other catecholamine-containing neuronal groups of the rat brain stem. The four NADP-dependent dehydrogenases were studied by both quantitative and qualitative histochemical techniques using adjacent tissue sections. Both types of analyses were done on neuronal nuclei known to contain catecholamines in high concentration, the nucleus locus ceruleus and the superior cervical sympathetic ganglion; other known catecholamine-containing nuclei were surveyed by the cytologic technique only. There was intense staining of cell bodies and neuropil of the nucleus locus ceruleus after all four of the NADP dehydrogenase histochemical reactions. In contrast, little staining was observed in the adjacent vestibular nuclei or mesencephalic root nucleus of the trigeminal with the exception of appreciable glucose 6-phosphate dehydrogenase activity present in neuropil elements. Quantitative microchemical determinations in the nucleus locus ceruleus corroborate the histochemical results which indicated high NADP dehydrogenase activities particularly for 6-phosphogluconate dehydrogenase. This same pattern of high NADP enzyme activity as determined by combined cytochemical and quantitative chemical techniques was also observed in the superior cervical ganglion and cytochemically in other catecholamine-containing nuclei of the brain stem. Our findings suggest that a high capacity to generate or utilize nicotinamide adenine dinucleotide phosphate (reduced) may be characteristic of those neurons which either receive adrenergic terminals or synthesize catecholamines.

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