Enhanced multiplication and progesterone production by porcine granulosa cells cultured in a serum-free medium

1981 ◽  
Vol 131 (2) ◽  
pp. 430-435 ◽  
Author(s):  
S. Stokłosowa ◽  
E. Gregoraszczuk ◽  
E. Holečková ◽  
J. Michl
Keyword(s):  
Granulosa Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Progesterone Production ◽  
Porcine Granulosa Cells ◽  
Cells Cultured

Effect of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells

1993 ◽  
Vol 129 (2) ◽  
pp. 165-168 ◽  
Author(s):  
József Bódis ◽  
Hans R Tinneberg ◽  
Attila Török ◽  
Philippe Cledon ◽  
Volker Hanf ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Direct Action ◽  
Serum Free Medium ◽  
Free Medium ◽  
Vitro Fertilization ◽  
Serum Free ◽  
Human Granulosa Cells ◽  
Estradiol Secretion ◽  
Cells Cultured

The aim of this study was to explore the direct action of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells cultured in serum-free medium. Progesterone and estradiol production was measured in the presence and absence of noradrenaline, dopamine or propranolol using radioimmunoassays; statistical analysis was performed by analysis of variance and Newman-Keul's multiple range test. Twenty-six women aged 31±3 years undergoing in vitro fertilization and embryo transfer for infertility treatment at University Women's Hospital, University of Tübingen, Germany, took part in this study. Noradrenaline significantly inhibited progesterone production by human granulosa cells in a dose-related manner at a concentration of 10−4–10−6 mol/l. Dopamine significantly stimulated estradiol secretion by granulosa cells in an inverse dose-related manner. Both effects were blocked by propranolol. The results suggest that catecholaminergic actions switch over the steroid production of human granulosa cells cultured in serum-free medium from progesterone to estradiol.

Plasma lipoproteins stimulate progestin production by rat ovarian granulosa cells cultured in serum free medium

1980 ◽  
Vol 13 (9) ◽  
pp. 1009-1014 ◽  
Author(s):  
James R. schreiber ◽  
Aaron J.W. Hsueh ◽  
David B. Weinstein ◽  
Gregory F. Erickson
Keyword(s):  
Granulosa Cells ◽  
Plasma Lipoproteins ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Ovarian Granulosa Cells ◽  
Cells Cultured

Factors Controlling Proliferation and Progesterone Production by Bovine Granulosa Cells in Serum-Free Medium*

Endocrinology ◽  
1981 ◽  
Vol 109 (2) ◽  
pp. 409-420 ◽  
Author(s):  
NAPHTALI SAVION ◽  
GE-MING LUI ◽  
RICHARD LAHERTY ◽  
DENIS GOSPODAROWICZ
Keyword(s):  
Granulosa Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Progesterone Production

Growth requirements and long-term subcultivation of bovine granulosa cells cultured in a serum-free medium

1991 ◽  
Vol 27 (7) ◽  
pp. 578-584 ◽  
Author(s):  
Hiroyoshi Hoshi ◽  
Yuji Takagi ◽  
Keizo Kobayashi ◽  
Masakazu Onodera ◽  
Taneaki Oikawa
Keyword(s):  
Granulosa Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Growth Requirements ◽  
Cells Cultured
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