Forskolin inhibits the Gs-stimulated adenylate cyclase in rat ascites hepatoma AH66F cells

1989 ◽  
Vol 184 (1) ◽  
pp. 219-227 ◽  
Author(s):  
Ken-Ichi Miyamoto ◽  
Fujiko Sanae ◽  
Ryozo Koshiura ◽  
Takayuki Matsunaga ◽  
Takaaki Hasegawa ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma

scholarly journals Effect of mitomycin C on the activation of adenylate cyclase in rat ascites hepatoma AH130 cells.

1986 ◽  
Vol 9 (9) ◽  
pp. 778-782
Author(s):  
KENICHI MIYAMOTO ◽  
TAKAYUKI MATSUNAGA ◽  
FUJIKO SANAE ◽  
RYOZO KOSHIURA
Keyword(s):  
Adenylate Cyclase ◽  
Mitomycin C ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma

Changes of Adenylate Cyclase Activity in AH-130 Ascites Hepatoma of Yoshida Induced by Enrichment with Fatty Acids

1995 ◽  
Vol 213 (3) ◽  
pp. 853-860 ◽  
Author(s):  
R.A. Canuto ◽  
L. Paradisi ◽  
G. Muzio ◽  
M. Maggiora ◽  
G. Mengozzi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Adenylate Cyclase ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Ascites Hepatoma

scholarly journals Inhibition of P-Glycoprotein-Dependent Multidrug Resistance by an Isoquinolinesulfonamide Compound H-87 in Rat Ascites Hepatoma AH66 Cells.

1996 ◽  
Vol 19 (6) ◽  
pp. 886-889 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Tomoyoshi MINAMINO ◽  
Masaaki NOMURA ◽  
Shinya WAKUSAWA ◽  
Ken-ichi MIYAMOTO ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Ascites Hepatoma ◽  
P Glycoprotein ◽  
Rat Ascites Hepatoma

Cell surface heparan sulphate and adhesive property of sublines of rat ascites hepatoma AH7974

1988 ◽  
Vol 90 (4) ◽  
pp. 683-689 ◽  
Author(s):  
A. Kimura ◽  
T. Kawaguchi ◽  
T. Ono ◽  
A. Sakuma ◽  
Y. Yokoya ◽  
...  
Keyword(s):  
Cell Surface ◽  
Culture Medium ◽  
Heparan Sulphate ◽  
Soluble Form ◽  
Foetal Calf Serum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Ascites Hepatoma ◽  
Serum Free ◽  
Rat Ascites Hepatoma

Two variants (74AD and 74FL) established from rat ascites hepatoma AH7974 were examined for the production of glycosaminoglycans in culture. There was no difference between the adhesive (74AD) and the floating (74FL) variants in quantity of glycosaminoglycans produced by their cultivation in minimum essential medium supplemented with 10% foetal calf serum. However, they were distinctly different in the distribution patterns of heparan sulphate. In 74FL, about 70% of total heparan sulphate was found in the culture medium in soluble form, whereas in 74AD, only 7% was found in the medium and the rest was in the cell-substratum complex. In a serum-free medium, 74AD cells grew without adhering to the substratum. After cultivation, more than 90% of total heparan sulphate was found in the cell-associated fractions and the rest in the substratum fractions. No heparan sulphate was detected in the culture medium. On the other hand, 74FL cells released heparan sulphate to the serum-free medium as much as to the serum-containing medium. The increase in amount of heparan sulphate in the culture medium of 74FL cells was supposed to be caused by failure of the cells to deposit heparan sulphate at the cell surface and not caused by increased production. Cell-substratum adhesion mechanisms involving cell surface heparan sulphate (heparan sulphate proteoglycan) and some serum intermediate(s) are discussed for 74AD cells.

Sign in / Sign up

Export Citation Format

Share Document