Schistosoma mansoni: Use of a cloned ribosomal RNA gene probe to detect restriction fragment length polymorphisms in the intermediate host Biomphalaria glabrata

1991 ◽  
Vol 73 (3) ◽  
pp. 285-294 ◽  
Author(s):  
Matty Knight ◽  
Paul J. Brindley ◽  
Charles S. Richards ◽  
Fred A. Lewis
Keyword(s):  
Schistosoma Mansoni ◽  
Intermediate Host ◽  
Restriction Fragment ◽  
Ribosomal Rna ◽  
Fragment Length ◽  
Biomphalaria Glabrata ◽  
Gene Probe ◽  
Ribosomal Rna Gene ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Detection of additional restriction fragment length polymorphisms among the weakly virulent (nonaggressive) and highly virulent (aggressive) isolates of Leptosphaeria maculans

1995 ◽  
Vol 41 (12) ◽  
pp. 1135-1141 ◽  
Author(s):  
N. A. Patterson ◽  
M. Kapoor
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Southern Hybridization ◽  
Leptosphaeria Maculans ◽  
Restriction Fragment Length Polymorphisms ◽  
Additional Restriction ◽  
Gene Probe ◽  
Genetic Characteristics ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Isolates of Leptosphaeria maculans were analyzed for their genetic relatedness based on DNA restriction fragment length polymorphisms (RFLPs), employing as Southern hybridization probes a combination of heat shock responsive genes (hsp70 and hsp80 from Neurospora crassa), the cutinase gene of Magnaporthe grisea, and cloned genomic DNA sequences from a virulent strain. Southern hybridization analysis revealed a high frequency of DNA polymorphism. Restriction fragments generated by each enzyme-probe combination resulted in distinct banding patterns, clearly separating the isolates into two groups. The cutinase gene probe did not reveal any polymorphisms. Although the majority of the probes used displayed RFLP profiles unique to each group, a nonaggressive isolate, LmA, showed additional genetic characteristics in common with the virulent pathotype.Key words: Leptosphaeria, genomic libraries, RFLP.

Discrimination between Gyrodactylus salaris, G. derjavini and G. truttae (Platyhelminthes: Monogenea) using restriction fragment length polymorphisms and an oligonucleotide probe within the small subunit ribosomal RNA gene

Parasitology ◽  
1995 ◽  
Vol 111 (1) ◽  
pp. 87-94 ◽  
Author(s):  
C. O. Cunningham ◽  
D. M. McGillivray ◽  
K. MacKenzie ◽  
W. T. Melvin
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Rna ◽  
Fragment Length ◽  
Oligonucleotide Probe ◽  
Small Subunit ◽  
Restriction Fragment Length Polymorphisms ◽  
Small Subunit Ribosomal Rna ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
Restriction Fragment Length

SUMMARYThe small subunit ribosomal RNA (srRNA) gene was amplified from Gyrodactylus salaris using the polymerase chain reaction (PCR), cloned, and the complete gene sequence of 1966 bp determined. The V4 region of the srRNA gene was identified and amplified from single specimens of G. salaris, G. derjavini and G. truttae. Comparison of the V4 sequences from these three species revealed sequence differences from which restriction fragment length polymorphisms (RFLPs) were predicted and an oligonucleotide probe (GsV4) specific to G. salaris designed. Digestion of the amplified V4 region of the srRNA gene with Hae III and either Alw I, BstY I, Dde I or Mbo I provided a means of discriminating between G. salaris, G. derjavini and G. truttae. The GsV4 probe was used to detect the srRNA gene from G. salaris in Southern and dot blots of the amplified V4 region. The nucleotide sequences reported in this paper have been submitted to the EMBL Data Library under accession numbers Z26942 (G. salaris), Z35128 (G. derjavini) and Z35129 (G. truttae).

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