Restriction fragment length polymorphisms in the ribosomal RNA gene complex of isolates of the entomopathogenic fungus Metarhizium anisopliae

1995 ◽  
Vol 99 (4) ◽  
pp. 485-491 ◽  
Author(s):  
N.D. Pipe ◽  
D. Chandler ◽  
B.W. Bainbridge ◽  
J.B. Heale
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Rna ◽  
Fragment Length ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Restriction Fragment Length Polymorphisms ◽  
Gene Complex ◽  
Ribosomal Rna Gene ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Genetic polymorphisms in three subtilisin-like protease isoforms (Pr1A, Pr1B, and Pr1C) from Metarhizium strains

2000 ◽  
Vol 46 (12) ◽  
pp. 1138-1144 ◽  
Author(s):  
Michael J Bidochka ◽  
Michael J Melzer
Keyword(s):  
Gene Family ◽  
Genetic Polymorphisms ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Ecori Site ◽  
Length Polymorphisms ◽  
Related Group ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms (RFLP) were examined in three isoforms of a gene family encoding subtilisin-like proteases (Pr1A, Pr1B, and Pr1C) in several isolates of the entomopathogenic fungus Metarhizium anisopliae. RFLP variation was not observed in any of the Pr1 genes from isolates within the same genetically related group. Between genetically related groups and between isolates from disparate geographical areas, the greatest variation in RFLP patterns was observed for Pr1A. When variation does occur at Pr1B and Pr1C, it was generally observed at an EcoRI site. Metarhizium anisopliae var. majus strain 473 and a M. flavoviride isolate were most dissimilar in RFLP patterns at all Pr1 genes when compared to the M. anisopliae strains. We suggest that Pr1 genes represent a gene family of subtilisin-like proteases and that the Pr1A gene encodes for the ancestral subtilisin-like protease which has subsequently duplicated and rearranged within the genome.Key words: Metarhizium anisopliae, protease, RFLP, entomopathogen.

Discrimination between Gyrodactylus salaris, G. derjavini and G. truttae (Platyhelminthes: Monogenea) using restriction fragment length polymorphisms and an oligonucleotide probe within the small subunit ribosomal RNA gene

Parasitology ◽  
1995 ◽  
Vol 111 (1) ◽  
pp. 87-94 ◽  
Author(s):  
C. O. Cunningham ◽  
D. M. McGillivray ◽  
K. MacKenzie ◽  
W. T. Melvin
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Rna ◽  
Fragment Length ◽  
Oligonucleotide Probe ◽  
Small Subunit ◽  
Restriction Fragment Length Polymorphisms ◽  
Small Subunit Ribosomal Rna ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
Restriction Fragment Length

SUMMARYThe small subunit ribosomal RNA (srRNA) gene was amplified from Gyrodactylus salaris using the polymerase chain reaction (PCR), cloned, and the complete gene sequence of 1966 bp determined. The V4 region of the srRNA gene was identified and amplified from single specimens of G. salaris, G. derjavini and G. truttae. Comparison of the V4 sequences from these three species revealed sequence differences from which restriction fragment length polymorphisms (RFLPs) were predicted and an oligonucleotide probe (GsV4) specific to G. salaris designed. Digestion of the amplified V4 region of the srRNA gene with Hae III and either Alw I, BstY I, Dde I or Mbo I provided a means of discriminating between G. salaris, G. derjavini and G. truttae. The GsV4 probe was used to detect the srRNA gene from G. salaris in Southern and dot blots of the amplified V4 region. The nucleotide sequences reported in this paper have been submitted to the EMBL Data Library under accession numbers Z26942 (G. salaris), Z35128 (G. derjavini) and Z35129 (G. truttae).

Sign in / Sign up

Export Citation Format

Share Document