scholarly journals Epidemiological typing of Yersinia enterocolitica by analysis of restriction fragment length polymorphisms with a cloned ribosomal RNA gene

1990 ◽  
Vol 32 (3) ◽  
pp. 179-187 ◽  
Author(s):  
J. K. Andersen ◽  
N. A. Saunders
Keyword(s):  
Yersinia Enterocolitica ◽  
Restriction Fragment ◽  
Ribosomal Rna ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Epidemiological Typing ◽  
Ribosomal Rna Gene ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

scholarly journals The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni

1996 ◽  
Vol 117 (2) ◽  
pp. 233-244 ◽  
Author(s):  
C. J. Jackson ◽  
A. J. Fox ◽  
D. R. A. Wareing ◽  
D. N. Hutchinson ◽  
D. M. Jones
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Rrna Gene ◽  
Epidemiological Typing ◽  
Length Polymorphisms ◽  
Genotypic Analysis ◽  
Ofcampylobacter Jejuni ◽  
Reference Strains ◽  
Restriction Fragment Length

SummaryCampylobacter jejuniserogroup reference strains and collections of sporadic and outbreak- associated isolates were examined for restriction fragment length polymorphisms (RFLPs), usingC. jejunirandom chromosomal and 16S rRNA gene probes. A collection of 48 Penner (HS) and 14 Lior (HL) serogroup reference strains, plus 10 clinical isolates, generated 35 RFLP and 26 ribotype patterns. In combination the two loci generated 48 distinct genotypes. Both probes were able to differentiate between certain random isolates of the same HS/HL serogroups but greater discrimination was obtained with RFLP than with ribotyping. Genotyping distinguished accurately between related and unrelated strains when applied to several outbreaks. Genotypic analysis ofC. jejuniby restriction fragment length polymorphisms is a valuable technique for epidemiological typing. Chromosomal variation detected by the two unlinked probe loci provides some information about the genetic relationship between isolates.

Discrimination between Gyrodactylus salaris, G. derjavini and G. truttae (Platyhelminthes: Monogenea) using restriction fragment length polymorphisms and an oligonucleotide probe within the small subunit ribosomal RNA gene

Parasitology ◽  
1995 ◽  
Vol 111 (1) ◽  
pp. 87-94 ◽  
Author(s):  
C. O. Cunningham ◽  
D. M. McGillivray ◽  
K. MacKenzie ◽  
W. T. Melvin
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Rna ◽  
Fragment Length ◽  
Oligonucleotide Probe ◽  
Small Subunit ◽  
Restriction Fragment Length Polymorphisms ◽  
Small Subunit Ribosomal Rna ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
Restriction Fragment Length

SUMMARYThe small subunit ribosomal RNA (srRNA) gene was amplified from Gyrodactylus salaris using the polymerase chain reaction (PCR), cloned, and the complete gene sequence of 1966 bp determined. The V4 region of the srRNA gene was identified and amplified from single specimens of G. salaris, G. derjavini and G. truttae. Comparison of the V4 sequences from these three species revealed sequence differences from which restriction fragment length polymorphisms (RFLPs) were predicted and an oligonucleotide probe (GsV4) specific to G. salaris designed. Digestion of the amplified V4 region of the srRNA gene with Hae III and either Alw I, BstY I, Dde I or Mbo I provided a means of discriminating between G. salaris, G. derjavini and G. truttae. The GsV4 probe was used to detect the srRNA gene from G. salaris in Southern and dot blots of the amplified V4 region. The nucleotide sequences reported in this paper have been submitted to the EMBL Data Library under accession numbers Z26942 (G. salaris), Z35128 (G. derjavini) and Z35129 (G. truttae).

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