EGF inhibits glucagon stimulation of amino acid transport in primary cultures of adult rat hepatocytes

FEBS Letters ◽  
1981 ◽  
Vol 127 (1) ◽  
pp. 109-111 ◽  
Author(s):  
Odette Morin ◽  
Claude Forest ◽  
Max Fehlmann
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Acid Transport ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Stimulation Of

scholarly journals Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin.

1976 ◽  
Vol 251 (10) ◽  
pp. 3014-3020 ◽  
Author(s):  
R F Kletzien ◽  
M W Pariza ◽  
J E Becker ◽  
V R Potter ◽  
F R Butcher
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Amino Acid Transport ◽  
Primary Cultures ◽  
Acid Transport ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Parenchymal Cells

scholarly journals Effects of growth factors on hormonal stimulation of amino acid transport in primary cultures of rat hepatocytes

1983 ◽  
Vol 210 (2) ◽  
pp. 361-366 ◽  
Author(s):  
P Auberger ◽  
M Samson ◽  
A Le Cam
Keyword(s):  
Growth Factors ◽  
Amino Acid ◽  
Growth Factor ◽  
Cyclic Amp ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Acid Transport ◽  
Hormonal Stimulation ◽  
Hormonal Effect ◽  
Stimulation Of

In primary cultures of rat hepatocytes, epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and foetal-calf serum (FCS) prevented the stimulation of amino acid transport by glucagon (cyclic AMP-dependent) and by catecholamines (cyclic AMP-independent), but not by insulin. The insulin effect, as well as the effect of other hormones, were totally inhibited by thrombin through a mechanism independent of its proteolytic activity. The inhibitory effect of growth factors, not found in freshly isolated hepatocytes, was expressed very early in culture (4h). Induction of tyrosine aminotransferase by glucagon or dexamethasone, which, like stimulation of transport, represents a late hormonal effect, was not affected by EGF, PDGF or FCS, but was inhibited by thrombin. In contrast, none of the rapid changes in protein phosphorylation caused by hormones was altered by growth factors. Thus the inhibition by growth factors of hormonal stimulation of transport presumably involves late step(s) in the cascade of events implicated in this hormonal effect.

Stimulation of DNA synthesis in primary cultures of adult rat hepatocytes by rat platelet-associated substance(s)

In Vitro ◽  
1982 ◽  
Vol 18 (2) ◽  
pp. 108-116 ◽  
Author(s):  
Alastair J. Strain ◽  
Joan A. McGowan ◽  
Nancy L. R. Bucher
Keyword(s):  
Dna Synthesis ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Stimulation Of
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