scholarly journals Partial purification of the D-glucose transport protein from human erythrocyte membranes by affinity chromatography on wheat germ lectin-sepharose

FEBS Letters ◽  
1981 ◽  
Vol 129 (1) ◽  
pp. 100-104 ◽  
Author(s):  
Gunnar Fröman ◽  
Per Lundahl ◽  
Fernando Acevedo
Keyword(s):  
Affinity Chromatography ◽  
Glucose Transport ◽  
Human Erythrocyte ◽  
Wheat Germ ◽  
Transport Protein ◽  
Erythrocyte Membranes ◽  
Partial Purification ◽  
Human Erythrocyte Membranes ◽  
Glucose Transport Protein ◽  
Wheat Germ Lectin

Conformation and stability of the anion transport protein of human erythrocyte membranes

Biochemistry ◽  
1985 ◽  
Vol 24 (12) ◽  
pp. 2843-2848 ◽  
Author(s):  
Kimio Oikawa ◽  
Debra M. Lieberman ◽  
Reinhart A. F. Reithmeier
Keyword(s):  
Human Erythrocyte ◽  
Anion Transport ◽  
Transport Protein ◽  
Erythrocyte Membranes ◽  
Human Erythrocyte Membranes

Evidence against the Involvement of Mutarotase in the D-Glucose Uptake Activity of Isolated Human Erythrocyte Membranes

1972 ◽  
Vol 50 (9) ◽  
pp. 1028-1030 ◽  
Author(s):  
Arthur Kahlenberg ◽  
Gary Miller
Keyword(s):  
Glucose Uptake ◽  
Membrane Transport ◽  
Glucose Transport ◽  
Human Erythrocyte ◽  
Transport Process ◽  
Human Erythrocytes ◽  
Erythrocyte Membranes ◽  
Human Erythrocyte Membranes ◽  
Uptake Activity ◽  
Glucose Carrier

Mutarotase, the enzyme catalyzing the interconversion of the anomeric forms of D-glucose, has recently been suggested to be the membrane glucose carrier in human erythrocytes. However, hemoglobin-free human erythrocyte membranes possessing D-glucose uptake activity were found to be free of mutarotase activity. Mutarotase activity was detected in the membrane-free hemolysates of the cells. It is therefore concluded that the D-glucose uptake activity of isolated erythrocyte membranes is not due to the binding of the sugar to mutarotase, and that this enzyme is not involved in glucose transport in a manner compatible with most presently held concepts of the membrane transport process.

Sign in / Sign up

Export Citation Format

Share Document