scholarly journals The 3′-terminal untranslated region of alfalfa mosaic virus RNA 4 facilitates the RNA entry into translation in a cell-free system

FEBS Letters ◽  
1993 ◽  
Vol 326 (1-3) ◽  
pp. 264-266 ◽  
Author(s):  
Lyubov A. Ryabova ◽  
Aleksey F. Torgashov ◽  
Oleg V. Kurnasov ◽  
Michail G. Bubunenko ◽  
Alexander S. Spirin
Keyword(s):  
Mosaic Virus ◽  
Untranslated Region ◽  
Alfalfa Mosaic Virus ◽  
Free System ◽  
Cell Free System ◽  
Virus Rna ◽  
A Cell

Tobacco mosaic virus RNA directs the synthesis of a coat protein peptide in a cell-free system from wheat

1973 ◽  
Vol 80 (4) ◽  
pp. 733-742 ◽  
Author(s):  
Bryan E. Roberts ◽  
Michael B. Mathews ◽  
Christopher J. Bruton
Keyword(s):  
Coat Protein ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Free System ◽  
Cell Free System ◽  
Virus Rna ◽  
A Cell

scholarly journals Authentic Replication and Recombination of Tomato Bushy Stunt Virus RNA in a Cell-Free Extract from Yeast

2008 ◽  
Vol 82 (12) ◽  
pp. 5967-5980 ◽  
Author(s):  
Judit Pogany ◽  
Peter D. Nagy
Keyword(s):  
High Efficiency ◽  
Tomato Bushy Stunt Virus ◽  
Free System ◽  
Cell Free System ◽  
Virus Rna ◽  
A Cell ◽  
Viral Replicase ◽  
Template Specificity ◽  
Rna Complex

ABSTRACT To study the replication of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we have developed a cell-free system based on a Saccharomyces cerevisiae extract. The cell-free system was capable of performing a complete replication cycle on added plus-stranded TBSV replicon RNA (repRNA) that led to the production of ∼30-fold-more plus-stranded progeny RNAs than the minus-stranded replication intermediate. The cell-free system also replicated the full-length TBSV genomic RNA, which resulted in production of subgenomic RNAs as well. The cell-free system showed high template specificity, since a mutated repRNA, minus-stranded repRNA, or a heterologous viral RNA could not be used as templates by the tombusvirus replicase. Similar to the in vivo situation, replication of the TBSV replicon RNA took place in a membraneous fraction, in which the viral replicase-RNA complex was RNase and protease resistant but sensitive to detergents. In addition to faithfully replicating the TBSV replicon RNA, the cell-free system was also capable of generating TBSV RNA recombinants with high efficiency. Altogether, tombusvirus replicase in the cell-free system showed features remarkably similar to those of the in vivo replicase, including carrying out a complete cycle of replication, high template specificity, and the ability to recombine efficiently.

scholarly journals The 5′ Untranslated Region of Alfalfa Mosaic Virus RNA 1 Is Involved in Negative-Strand RNA Synthesis

2003 ◽  
Vol 77 (20) ◽  
pp. 11284-11289 ◽  
Author(s):  
A. Corina Vlot ◽  
John F. Bol
Keyword(s):  
Mosaic Virus ◽  
Untranslated Region ◽  
Rna Synthesis ◽  
Alfalfa Mosaic Virus ◽  
Loop Structure ◽  
Stem Loop ◽  
Genomic Rnas ◽  
Negative Strand ◽  
Stem Loop Structure ◽  
Virus Rna

ABSTRACT The three genomic RNAs of alfalfa mosaic virus each contain a unique 5′ untranslated region (5′ UTR). Replacement of the 5′ UTR of RNA 1 by that of RNA 2 or 3 yielded infectious replicons. The sequence of a putative 5′ stem-loop structure in RNA 1 was found to be required for negative-strand RNA synthesis. A similar putative 5′ stem-loop structure is present in RNA 2 but not in RNA 3.

Cauliflower mosaic virus-induced viroplasms support viral DNA synthesis in a cell-free system

Virology ◽  
1984 ◽  
Vol 133 (2) ◽  
pp. 289-300 ◽  
Author(s):  
N. Modjtahedi ◽  
M. Volovitch ◽  
L. Sossountzov ◽  
Y. Habricot ◽  
J.M. Bonneville ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Viral Dna ◽  
Free System ◽  
Cell Free System ◽  
A Cell
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