scholarly journals Structural and functional characterization of cytochrome c 3 from D. desulfuricans ATCC 27774 by 1 H-NMR

FEBS Letters ◽  
1996 ◽  
Vol 390 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Ricardo O. Louro ◽  
Isabel Pacheco ◽  
David L. Turner ◽  
Jean LeGall ◽  
António V. Xavier
Keyword(s):  
Cytochrome C ◽  
Functional Characterization ◽  
H Nmr

scholarly journals Functional characterization of a pyrimidine-rich element in the 5'-noncoding region of the yeast iso-1-cytochrome c gene.

1988 ◽  
Vol 8 (3) ◽  
pp. 1045-1054 ◽  
Author(s):  
J B McNeil
Keyword(s):  
Cytochrome C ◽  
Gene Fusion ◽  
Sequence Data ◽  
Functional Characterization ◽  
Noncoding Region ◽  
Start Site ◽  
Transcription Start ◽  
Template Strand ◽  
Tata Element

A predominantly pyrimidine-rich sequence (purine in the template strand, 32 of 37 bases) is located between a functional TATA element and the corresponding transcription start site region of the Saccharomyces cerevisiae iso-1-cytochrome c (CYC1) gene. By using linker deletions and gene fusion techniques, the functional characteristics of this pyrimidine sequence were examined. Results indicate that the function of this element is to limit the accumulation of full-length mRNAs with 5' ends which map upstream of the pyrimidine-rich sequence. Data suggest that the 5'-noncoding region of the CYC1 gene possesses signals for mRNA 3'-end processing.

scholarly journals Reconstitution of cytochrome c oxidase in phospholipid vesicles containing polyvinylic polymers

1989 ◽  
Vol 257 (3) ◽  
pp. 783-787 ◽  
Author(s):  
P Sarti ◽  
G Antonini ◽  
F Malatesta ◽  
B Vallone ◽  
S Villaschi ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Functional Characterization ◽  
Respiratory Control ◽  
Phospholipid Vesicles ◽  
Lipid Phase ◽  
Protein Orientation ◽  
Highly Hydrophobic ◽  
Polymer Interaction

Cytochrome c oxidase was reconstituted in phospholipid vesicles in the presence of highly hydrophobic poly(vinyl alkanoate) polymers. Electron-microscopy observations demonstrated that polymer interaction with the lipid phase induces vesicles to adopt smaller diameters than those typical of standard proteoliposomes. Functional characterization of these polymer-proteoliposome structures indicates that the reconstitution of the enzyme proceeds efficiently without causing either scrambling of the protein orientation in the membrane or loss of respiratory control. A clear dependence of respiratory control ratio on vesicle size was also demonstrated, which is in agreement with a previous model proposed for control of activity of cytochrome c oxidase vesicles [Brunori, Sarti, Colosimo, Antonini, Malatesta, Jones & Wilson (1985) EMBO J. 4, 2365-2368].

Functional characterization of a pyrimidine-rich element in the 5'-noncoding region of the yeast iso-1-cytochrome c gene

1988 ◽  
Vol 8 (3) ◽  
pp. 1045-1054
Author(s):  
J B McNeil
Keyword(s):  
Cytochrome C ◽  
Gene Fusion ◽  
Sequence Data ◽  
Functional Characterization ◽  
Noncoding Region ◽  
Start Site ◽  
Transcription Start ◽  
Template Strand ◽  
Tata Element

A predominantly pyrimidine-rich sequence (purine in the template strand, 32 of 37 bases) is located between a functional TATA element and the corresponding transcription start site region of the Saccharomyces cerevisiae iso-1-cytochrome c (CYC1) gene. By using linker deletions and gene fusion techniques, the functional characteristics of this pyrimidine sequence were examined. Results indicate that the function of this element is to limit the accumulation of full-length mRNAs with 5' ends which map upstream of the pyrimidine-rich sequence. Data suggest that the 5'-noncoding region of the CYC1 gene possesses signals for mRNA 3'-end processing.

scholarly journals Structural and functional characterization of phosphomimetic mutants of cytochrome c at threonine 28 and serine 47

2016 ◽  
Vol 1857 (4) ◽  
pp. 387-395 ◽  
Author(s):  
Alejandra Guerra-Castellano ◽  
Irene Díaz-Moreno ◽  
Adrián Velázquez-Campoy ◽  
Miguel A. De la Rosa ◽  
Antonio Díaz-Quintana
Keyword(s):  
Cytochrome C ◽  
Functional Characterization

Structural and functional characterization of CcmG from Pseudomonas aeruginosa, a key component of the bacterial cytochrome c maturation apparatus

2010 ◽  
Vol 78 (10) ◽  
pp. 2213-2221 ◽  
Author(s):  
Adele Di Matteo ◽  
Nicoletta Calosci ◽  
Stefano Gianni ◽  
Per Jemth ◽  
Maurizio Brunori ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cytochrome C ◽  
Functional Characterization ◽  
Cytochrome C Maturation

scholarly journals Spectroscopic, Structural, and Functional Characterization of the Alternative Low-Spin State of Horse Heart Cytochrome c

2008 ◽  
Vol 94 (10) ◽  
pp. 4066-4077 ◽  
Author(s):  
Katia C.U. Mugnol ◽  
Rômulo A. Ando ◽  
Rafael Y. Nagayasu ◽  
Adelaide Faljoni-Alario ◽  
Sergio Brochsztain ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Spin State ◽  
Functional Characterization ◽  
Horse Heart Cytochrome ◽  
Horse Heart Cytochrome C

scholarly journals Signatures Of Tspan8 Variants Associated With Human Metabolic Regulation And Diseases

2020 ◽  
Author(s):  
Tisham De ◽  
Angela Goncalves ◽  
Doug Speed ◽  
Phillipe Froguel ◽  
Daniel Gaffney ◽  
...  
Keyword(s):  
Metabolic Regulation ◽  
Metabolic Diseases ◽  
Functional Characterization ◽  
H Nmr ◽  
Number Variation ◽  
Exon 11 ◽  
Induced Pluripotent ◽  
Obesity And Cancer ◽  
Complex Human Traits

AbstractHere, with the example of common copy number variation (CNV) in the TSPAN8 gene, we present an important piece of work in the field of CNV detection, CNV association with complex human traits such as 1H NMR metabolomic phenotypes and an example of functional characterization of CNVs among human induced pluripotent stem cells (HipSci). We report TSPAN8 exon 11 as a new locus associated with metabolomic regulation and show that its biology is associated with several metabolic diseases such as type 2 diabetes (T2D), obesity and cancer. Our results further demonstrate the power of multivariate association models over univariate methods and define new metabolomic signatures for several new genomic loci, which can act as a catalyst for new diagnostics and therapeutic approaches.

Biochemical and functional characterization of a periplasmic disulfide oxidoreductase from Neisseria meningitidis essential for meningococcal viability

2015 ◽  
Vol 468 (2) ◽  
pp. 271-282 ◽  
Author(s):  
Adeline Gand ◽  
Laure Selme-Roussel ◽  
Sabrina Collin ◽  
Guy Branlant ◽  
Christophe Jacob ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Neisseria Meningitidis ◽  
In Silico ◽  
Functional Characterization ◽  
Maturation Process ◽  
Cytochrome C Maturation ◽  
Disulfide Oxidoreductase

Using in silico, biochemical, enzymatic and genetic approaches, we have identified and characterized a new periplasmic thioredoxin-like protein from Neisseria meningitidis probably involved in the cytochrome c maturation process and essential for the pathogen viability

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