Analysis of recombinant human growth hormone in Escherichia coli fermentation broth by micellar high-performance liquid chromatography

1995 ◽  
Vol 705 (1) ◽  
pp. 155-161 ◽  
Author(s):  
Mark A. Strege ◽  
Avinash L. Lagu
Keyword(s):  
Escherichia Coli ◽  
Growth Hormone ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Growth Hormone ◽  
High Performance ◽  
Fermentation Broth ◽  
Recombinant Human Growth Hormone ◽  
Human Growth

scholarly journals Determination of human growth hormone in pure and pharmaceutical dosage form by spectrofluorometry and high performance liquid chromatography

2012 ◽  
Vol 18 (3) ◽  
pp. 399-405 ◽  
Author(s):  
Bilal Yilmaz ◽  
Yucel Kadioglu ◽  
Kadem Meral ◽  
Yavuz Onganer
Keyword(s):  
Growth Hormone ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Growth Hormone ◽  
High Performance ◽  
Dosage Form ◽  
Human Growth ◽  
Pharmaceutical Dosage Form ◽  
Relative Standard

In this study, a new and rapid spectrofluorometry and high performance liquid chromatography (HPLC) methods were developed for determination of human growth hormone in pure and pharmaceutical dosage form. The solvent system, wavelength of detection and chromatographic conditions were optimized in order to maximize the sensitivity of both the proposed methods. The linearity was established over the concentration range of 1.25-50?g mL-1 for spectrofluorometry and 10-75?g mL-1 for HPLC method. The intra- and inter-day relative standard deviation (RSD) was less than 8.46 and 5.98% for spectrofluorometry and HPLC, respectively. Limits of quantitation were determined as 0.075 and 7.5?g mL-1 for spectrofluorometry and HPLC, respectively. No interference was found from tablet excipients at the selected assay conditions. The methods were applied for the quality control of commercial growth hormone dosage form to quantify the drug and to check the formulation content uniformity.

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