Electrically controlled ion transfer and pH change near a conducting polymer-coated electrode

1989 ◽  
Vol 266 (2) ◽  
pp. 297-308 ◽  
Author(s):  
Hiroaki Shinohara ◽  
Junichiro Kojima ◽  
Masuo Aizawa
2012 ◽  
Vol 90 (3) ◽  
pp. 271-277 ◽  
Author(s):  
Hakim Mehenni

In this study, a simple and direct biosensor was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the determination of avidin, a highly stable glycoprotein found in egg whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on poly-3′-(2-methoxy-5-amino-β-styryl)-(2,2′:5′,2″-terthiophene) (PMAST), and the biotin–avidin interaction was monitored by square-wave voltammetry. Incubation of the PMAST/biotin-modified coated electrode with avidin in a phosphate-buffered saline solution caused a significant change to its square-wave voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to determine avidin. Importantly, we found a linear relationship for the avidin sensor in the range of 4 × 10−14 to 3 × 10−4 mol/L, and the detection limit was determined to be approximately 10−14 mol/L.


2014 ◽  
Vol 30 (3) ◽  
pp. 351-357 ◽  
Author(s):  
Yumi YOSHIDA ◽  
Junya UCHIDA ◽  
Shotaro NAKAMURA ◽  
Satoshi YAMAGUCHI ◽  
Kohji MAEDA

2012 ◽  
Vol 65 (4) ◽  
pp. 395 ◽  
Author(s):  
Hakim Mehenni ◽  
Lê H. Dao

In this study, a simple and direct biodetector was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the detection of avidin, a highly stable glycoprotein found in egg-whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on the poly 3′-(3-chloro-4-amino-β-styryl)-(2,2′ : 5′,2″-terthiophene) (PCAST), and the biotin–avidin interaction was monitored by cyclic voltammetry. Incubation of the PCAST/biotin-modified-coated electrode with avidin in a phosphate buffered saline solution caused a significant change to its cyclic voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to detect avidin at 4 × 10–6 mol L–1.


1989 ◽  
Vol 276 (1) ◽  
pp. 23-35 ◽  
Author(s):  
Hiroaki Shinohara ◽  
Junichiro Kojima ◽  
Masashi Yaoita ◽  
Masuo Aizawa

1989 ◽  
Vol 22 (1) ◽  
pp. 23-35 ◽  
Author(s):  
Hiroaki Shinohara ◽  
Junichiro Kojima ◽  
Masashi Yaoita ◽  
Masuo Aizawa

2011 ◽  
Vol 57 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Yumi Yoshida ◽  
Satoshi Yamaguchi ◽  
Shotaro Nakamura ◽  
Kohji Maeda

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