Towards the Development of a Direct Electrochemical Biodetector of Avidin Based on the Poly(chloro amino-β-styryl terthiophene)-Coated Glassy Carbon Electrode

2012 ◽  
Vol 65 (4) ◽  
pp. 395 ◽  
Author(s):  
Hakim Mehenni ◽  
Lê H. Dao
Keyword(s):  
Cyclic Voltammetry ◽  
Conducting Polymer ◽  
Glassy Carbon ◽  
Saline Solution ◽  
Cyclic Voltammogram ◽  
Carbon Electrode ◽  
Ion Transfer ◽  
Amine Group ◽  
Coated Electrode ◽  
Phosphate Buffered Saline

In this study, a simple and direct biodetector was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the detection of avidin, a highly stable glycoprotein found in egg-whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on the poly 3′-(3-chloro-4-amino-β-styryl)-(2,2′ : 5′,2″-terthiophene) (PCAST), and the biotin–avidin interaction was monitored by cyclic voltammetry. Incubation of the PCAST/biotin-modified-coated electrode with avidin in a phosphate buffered saline solution caused a significant change to its cyclic voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to detect avidin at 4 × 10–6 mol L–1.

Development of an avidin sensor based on the poly(methoxy amino-β-styryl terthiophene)-coated glassy carbon electrode

2012 ◽  
Vol 90 (3) ◽  
pp. 271-277 ◽  
Author(s):  
Hakim Mehenni
Keyword(s):  
Conducting Polymer ◽  
Square Wave Voltammetry ◽  
Saline Solution ◽  
Carbon Electrode ◽  
Ion Transfer ◽  
Square Wave ◽  
Wave Voltammetry ◽  
Coated Electrode ◽  
Phosphate Buffered Saline

In this study, a simple and direct biosensor was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the determination of avidin, a highly stable glycoprotein found in egg whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on poly-3′-(2-methoxy-5-amino-β-styryl)-(2,2′:5′,2″-terthiophene) (PMAST), and the biotin–avidin interaction was monitored by square-wave voltammetry. Incubation of the PMAST/biotin-modified coated electrode with avidin in a phosphate-buffered saline solution caused a significant change to its square-wave voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to determine avidin. Importantly, we found a linear relationship for the avidin sensor in the range of 4 × 10−14 to 3 × 10−4 mol/L, and the detection limit was determined to be approximately 10−14 mol/L.

scholarly journals Investigation of Electrochemical Behaviour of Quercetin on the Modified Electrode Surfaces with Procaine and Aminophenyl in Non-Aquous Medium

2008 ◽  
Vol 5 (3) ◽  
pp. 539-550 ◽  
Author(s):  
Ibrahim Ender Mulazimoglu ◽  
Erdal Ozkan
Keyword(s):  
Cyclic Voltammetry ◽  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Glassy Carbon ◽  
Electrode Surface ◽  
Electrochemical Impedance ◽  
Electrochemical Behaviour ◽  
Carbon Electrode ◽  
Amine Group ◽  
Tetrabutylammonium Tetrafluoroborate

In this study, cyclic voltammetry and electrochemical ımpedance spectroscopy have been used to investigate the electrochemical behaviour of quercetin (3,3′,4′,5,7-pentahydroxyflavone) on the procaine and aminophenyl modified electrode. The modification of procaine and aminophenyl binded electrode surface with quercetin was performed in +0,3/+2,8 V (for procaine) and +0,4/+1,5 V (for aminophenyl) potential range using 100 mV s-1scanning rate having 10 cycle. A solution of 0.1 M tetrabutylammonium tetrafluoroborate in acetonitrile was used as a non-aquous solvent. For the modification process a solution of 1 mM quercetin in 0.1 M tetrabutylammonium tetrafluoroborate was used. In order to obtain these two surface, a solution of 1 mM procaine and 1 mM nitrophenyl diazonium salt in 0.1 M tetrabutylammonium tetrafluoroborate was used. By using these solutions bare glassy carbon electrode surface was modified. Nitrophenyl was reduced to amine group in 0.1 M HCl medium on the nitrophenyl modified glassy carbon elelctrode surface. Procaine modified glassy carbon electrode surface was quite electroactive. Although nitrophenyl modified glassy carbon elelctrode surface was electroinactive, it was activated by reducing nitro group into amine group. For the characterization of the modified surface 1 mM ferrocene in 0.1 M tetrabutylammonium tetrafluoroborate for cyclic voltammetry and 1 mM ferricyanide/ferrocyanide (1:1) mixture in 0,1 M KCl for electrochemical impedance spectroscopy were used.

Solar Exfoliated Graphene Oxide: A Platform for Electrochemical Sensing of Epinephrine

2020 ◽  
Vol 16 (4) ◽  
pp. 393-403 ◽  
Author(s):  
Renjini Sadhana ◽  
Pinky Abraham ◽  
Anithakumary Vidyadharan
Keyword(s):  
Cyclic Voltammetry ◽  
Graphene Oxide ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Simultaneous Detection ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Reduced Graphene ◽  
Pulse Voltammetry

Introduction: In this study, solar exfoliated graphite oxide modified glassy carbon electrode was used for the anodic oxidation of epinephrine in a phosphate buffer medium at pH7. The modified electrode showed fast response and sensitivity towards Epinephrine Molecule (EP). The electrode was characterized electrochemically through Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). Area of the electrode enhanced three times during modification and studies reveal that the oxidation process of EP occurs by an adsorption controlled process involving two electrons. The results showed a detection limit of 0.50 ± 0.01μM with a linear range up to 100 μM. The rate constant calculated for the electron transfer reaction is 1.35 s-1. The electrode was effective for simultaneous detection of EP in the presence of Ascorbic Acid (AA) and Uric Acid (UA) with well-resolved signals. The sensitivity, selectivity and stability of the sensor were also confirmed. Methods: Glassy carbon electrode modified by reduced graphene oxide was used for the detection and quantification of epinephrine using cyclic voltammetry and differential pulse voltammetry. Results: The results showed an enhancement in the electrocatalytic oxidation of epinephrine due to the increase in the effective surface area of the modified electrode. The anodic transfer coefficient, detection limit and electron transfer rate constant of the reaction were also calculated. Conclusion: The paper reports the determination of epinephrine using reduced graphene oxide modified glassy carbon electrode through CV and DPV. The sensor exhibited excellent reproducibility and repeatability for the detection of epinephrine and also its simultaneous detection of ascorbic acid and uric acid, which coexist in the biological system.

scholarly journals Electrocatalytic Study of Paracetamol at a Single-Walled Carbon Nanotube/Nickel Nanocomposite Modified Glassy Carbon Electrode

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Koh Sing Ngai ◽  
Wee Tee Tan ◽  
Zulkarnain Zainal ◽  
Ruzniza Mohd Zawawi ◽  
Joon Ching Juan
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Nanotube ◽  
Glassy Carbon Electrode ◽  
Modified Electrode ◽  
Glassy Carbon ◽  
Carbon Electrode ◽  
Single Walled Carbon Nanotube ◽  
Paracetamol Concentration ◽  
Single Walled Carbon

A rapid, simple, and sensitive method for the electrochemical determination of paracetamol was developed. A single-walled carbon nanotube/nickel (SWCNT/Ni) nanocomposite was prepared and immobilized on a glassy carbon electrode (GCE) surface via mechanical attachment. This paper reports the voltammetry study on the effect of paracetamol concentration, scan rate, pH, and temperature at a SWCNT/Ni-modified electrode in the determination of paracetamol. The characterization of the SWCNT/Ni/GCE was performed by cyclic voltammetry. Variable pressure scanning electron microscopy (VPSEM) and energy dispersive X-ray (EDX) spectrometer were used to examine the surface morphology and elemental profile of the modified electrode, respectively. Cyclic voltammetry showed significant enhancement in peak current for the determination of paracetamol at the SWCNT/Ni-modified electrode. A linear calibration curve was obtained for the paracetamol concentration between 0.05 and 0.50 mM. The SWCNT/Ni/GCE displayed a sensitivity of 64 mA M−1and a detection limit of 1.17 × 10−7 M in paracetamol detection. The proposed electrode can be applied for the determination of paracetamol in real pharmaceutical samples with satisfactory performance. Results indicate that electrodes modified with SWCNT and nickel nanoparticles exhibit better electrocatalytic activity towards paracetamol.

scholarly journals Electrochemical Oxidation of Paracetamol Mediated byMgB2Microparticles Modified Glassy Carbon Electrode

2011 ◽  
Vol 8 (2) ◽  
pp. 553-560 ◽  
Author(s):  
Mohammed Zidan ◽  
Tan Wee Tee ◽  
A. Halim Abdullah ◽  
Zulkarnain Zainal ◽  
Goh Joo Kheng
Keyword(s):  
Cyclic Voltammetry ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Supporting Electrolyte ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Diffusion Controlled ◽  
Scan Rate ◽  
Degree Of Stability ◽  
A Current

A MgB2microparticles modified glassy carbon electrode (MgB2/GCE) was fabricated by adhering microparticles of MgB2onto the electrode surface of GCE. It was used as a working electrode for the detection of paracetamol in 0.1 M KH2PO4aqueous solution during cyclic voltammetry. Use of the MgB2/GCE the oxidation process of paracetamol with a current enhancement significantly by about 2.1 times. The detection limit of this modified electrode was found to be 30 μM. The sensitivity under conditions of cyclic voltammetry is significantly dependent on pH, supporting electrolyte, temperature and scan rate. The current enhancement observed in different electrolytic media varied in the following order: KH2PO4> KCl > K2SO4> KBr. Interestingly, the oxidation of paracetamol using modified GC electrode remain constant even after 15 cycling. It is therefore evident that the MgB2modifiedGCelectrode possesses some degree of stability. A slope of 0.52 dependent of scan rate on current indicates that the system undergoes diffusion-controlled process.

Electrocatalytic Oxidation of Nitrite at Platinum Particles Modified Glassy Carbon Electrode

2012 ◽  
Vol 512-515 ◽  
pp. 2291-2294
Author(s):  
Yu Cui Hao ◽  
Li Shen ◽  
Zhi Ai
Keyword(s):  
Cyclic Voltammetry ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Electrocatalytic Oxidation ◽  
Influence Factors ◽  
Carbon Electrode ◽  
Nitrite Oxidation ◽  
Modified Glassy Carbon Electrode ◽  
Base Solution ◽  
Platinum Particles

Platinum particles modified glassy carbon electrode（Pt/GCE）was prepared by electrochemically deposite. The electrochemica1 behaviors of nitrite on Pt/GCE were studied by cyclic voltammetry. The influence factors of electrocatalysis for nitrite oxidation on Pt/GCE were studied. The result showed that Pt/GCE has favorable electrocatalysis for nitrite. oxidation, which was effected by the amount of platinum particles and the pH of base solution

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