Genomic organization and functional analysis of a deletion variant of the 87A7 heat shock locus of Drosophila melanogaster

1982 ◽  
Vol 155 (3) ◽  
pp. 267-280 ◽  
Author(s):  
Andor Udvardy ◽  
János Sümegi ◽  
Éva Csordás Tóth ◽  
János Gausz ◽  
Henrik Gyurkovics ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Genomic Organization ◽  
Deletion Variant ◽  
Shock Locus

scholarly journals MUTATIONAL ANALYSIS OF THE REGION SURROUNDING THE 93D HEAT SHOCK LOCUS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1984 ◽  
Vol 106 (2) ◽  
pp. 249-265
Author(s):  
Jym Mohler ◽  
Mary Lou Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Mutational Analysis ◽  
Point Mutations ◽  
Γ Irradiation ◽  
Lethal Mutations ◽  
Complementation Groups ◽  
In Trans ◽  
Shock Locus ◽  
Shock Proteins

ABSTRACT The region containing subdivisions 93C, 93D and 93E on chromosome 3 of Drosophila melanogaster has been screened for visible and lethal mutations. Treatment with three mutagens, γ irradiation, ethyl methanesulfonate and diepoxybutane, has produced mutations that fall into 20 complementation groups, including the previously identified ebony locus. No point mutations affecting the heat shock locus in 93D were detected; however, a pair of deficiencies that overlap in the region of this locus was isolated. Flies heterozygous in trans for this pair of deficiencies are capable of producing all of the major heat shock puffs (except 93D) and the major heat shock proteins. In addition, these flies show recovery of normal protein synthesis following a heat shock.

Deficiency mapping of the 93D heat-shock locus in Drosophila melanogaster

Chromosoma ◽  
1982 ◽  
Vol 86 (4) ◽  
pp. 457-467 ◽  
Author(s):  
Jym Mohler ◽  
Mary Lou Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Shock Locus

scholarly journals Genomic organization and transcription of the αβ heat shock DNA in Drosophila melanogaster

1981 ◽  
Vol 9 (20) ◽  
pp. 5297-5310 ◽  
Author(s):  
John T. Lis ◽  
David Ish-Horowicz ◽  
Sheena M. Pinchin
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Genomic Organization

scholarly journals GENETIC CHARACTERIZATION OF THE REGION BETWEEN 86F1,2 AND 87B15 ON CHROMOSOME 3 OF DROSOPHILA MELANOGASTER

Genetics ◽  
1981 ◽  
Vol 98 (4) ◽  
pp. 775-789
Author(s):  
J Gausz ◽  
H Gyurkovics ◽  
G Bencze ◽  
A A M Awad ◽  
J J Holden ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Genetic Characterization ◽  
Chromosome 3 ◽  
Gene Encoding ◽  
Lethal Mutations ◽  
Complementation Groups ◽  
Shock Locus ◽  
Chromosome Bands

ABSTRACT The region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster, which contains about 27 polytene chromosome bands including the 87A7 heat-shock locus, has been screened for EMS-induced visible and lethal mutations. We have recovered 268 lethal mutations that fall into 25 complementation groups. Cytogenetic localization of the complementation groups by deficiency mapping is consistent with the notion that each band encodes a single genetic function. We have also screened for mutations at the 87A7 heat shock locus, using a chromosome that has only one copy of the gene encoding the 70,000 dalton heat-shock protein (hsp70). No lethal or visible mutations at 87A7 were identified from 10,719 mutagenized chromosomes, and no female-sterile mutations at 87A7 were recovered from the 1,520 chromosomes whose progeny were tested for female fertility. We found no evidence that a functional hsp70 gene is required for development under laboratory conditions.

scholarly journals Sequence evolution of the Drosophila heat shock locus hsr omega. I. The nonrepeated portion of the gene.

Genetics ◽  
1989 ◽  
Vol 122 (2) ◽  
pp. 403-415 ◽  
Author(s):  
J C Garbe ◽  
W G Bendena ◽  
M L Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Sequence Evolution ◽  
Phenotypic Characteristics ◽  
Cross Hybridization ◽  
Protein Coding ◽  
Large Heat ◽  
Shock Locus ◽  
Shock Proteins

Abstract The locus which we now call hsr omega was originally identified as a large heat shock puff in polytene region 93D of Drosophila melanogaster. This puff was subsequently found to have several phenotypic characteristics that distinguished it from other heat shock puffs. These characteristics include induction by a number of agents that do not induce other puffs and the presence of large ribonucleotide particles that are not found elsewhere. Each Drosophila species has one heat shock puff with these phenotypes. In contrast to the strong sequence conservation seen in puffs coding for heat shock proteins, very little cross-hybridization is detected between hsr omega loci in different species, suggesting that the hsr omega loci are diverging rapidly. Comparative analyses of the hsr omega locus from D. melanogaster, D. pseudoobscura, and D. hydei show that, despite the sequence change, the structure of the locus and its transcripts has been conserved, along with a number of short regions of the sequence. The short regions of conservation offer some clues to the function of this unusual locus. In addition, these comparisons offer a view of the evolution of a gene whose primary function does not appear to be protein coding.

Regulated expression of a Drosophila melanogaster heat shock locus after stable integration in a Drosophila hydei cell line

1985 ◽  
Vol 5 (11) ◽  
pp. 3208-3213
Author(s):  
J H Sinclair ◽  
S E Saunders ◽  
J F Burke ◽  
J H Sang
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cell Line ◽  
Stable Integration ◽  
Drosophila Hydei ◽  
Regulated Expression ◽  
Shock Locus

DNA-mediated cotransformation has been used to transfer a Drosophila melanogaster heat shock locus into cultured Drosophila hydei cells by use of the copia-based selectable vector pCV2gpt and of pMH10A, a cloned 87A7 heat shock locus encoding a mutant heat shock protein (hsp). Transformed lines contain between 50 and 200 copies of both plasmids, each separately organized as a head-to-tail concatemer which is stably maintained in the transformed lines. Exposure of the cotransformants to heat shock temperatures induces the regulated expression of the hsp RNA and the mutant hsp in all the lines analyzed.

Sign in / Sign up

Export Citation Format

Share Document