Probing the assembly of the 3′ major domain of 16 S ribosomal RNA

1988 ◽  
Vol 200 (2) ◽  
pp. 309-319 ◽  
Author(s):  
Ted Powers ◽  
Li-ming Changchien ◽  
Gary R. Craven ◽  
Harry F. Noller
Keyword(s):  
RNA ◽  
1998 ◽  
Vol 4 (11) ◽  
pp. 1455-1466 ◽  
Author(s):  
ALEXANDRE MONTPETIT ◽  
CATHERINE PAYANT ◽  
JAMES M. NOLAN ◽  
LÉA BRAKIER-GINGRAS

1996 ◽  
Vol 259 (2) ◽  
pp. 207-215 ◽  
Author(s):  
François Dragon ◽  
Catherine Spickler ◽  
Robert Pinard ◽  
Julie Carrière ◽  
Léa Brakier-Gingras

Author(s):  
Daniel Beniac ◽  
George Harauz

The structures of E. coli ribosomes have been extensively probed by electron microscopy of negatively stained and frozen hydrated preparations. Coupled with quantitative image analysis and three dimensional reconstruction, such approaches are worthwhile in defining size, shape, and quaternary organisation. The important question of how the nucleic acid and protein components are arranged with respect to each other remains difficult to answer, however. A microscopical technique that has been proposed to answer this query is electron spectroscopic imaging (ESI), in which scattered electrons with energy losses characteristic of inner shell ionisations are used to form specific elemental maps. Here, we report the use of image sorting and averaging techniques to determine the extent to which a phosphorus map of isolated ribosomal subunits can define the ribosomal RNA (rRNA) distribution within them.


Sign in / Sign up

Export Citation Format

Share Document