In vitro studies on steroid metabolism of testicular tissue in ambisexual teleost fish

1975 ◽  
Vol 6 (3-4) ◽  
pp. 341-344 ◽  
Author(s):  
Rudolf Reinboth
Keyword(s):  
Teleost Fish ◽  
Testicular Tissue ◽  
In Vitro Studies ◽  
Steroid Metabolism

STEROID METABOLISM OF A SERTOLI CELL TUMOUR OF THE TESTIS OF A DOG WITH FEMINIZATION AND ALOPECIA AND OF THE NORMAL CANINE TESTIS

1967 ◽  
Vol 38 (1) ◽  
pp. 61-NP ◽  
Author(s):  
C. G. PIERREPOINT ◽  
JEAN McI. GALLEY ◽  
K. GRIFFITHS ◽  
J. K. GRANT
Keyword(s):  
Sertoli Cell ◽  
Direct Evidence ◽  
Hydroxysteroid Dehydrogenase ◽  
Testicular Tissue ◽  
Cell Tumour ◽  
Tumour Tissue ◽  
Steroid Metabolism ◽  
Enzyme Systems ◽  
Sertoli Cell Tumour

SUMMARY The metabolism of [7α-3H]pregnenolone and [4-14C]dehydroepiandrosterone (DHA) by a Sertoli cell tumour of the testis from a dog with signs of feminization has been studied in vitro and compared with the metabolism of the normal canine testis. The tumour formed oestrone and oestradiol-17β from DHA thus providing direct evidence for the synthesis of oestrogen by this type of neoplasm. Relative or complete inactivity of several enzyme systems involved in the synthesis of testosterone was found in the tumour tissue, and the conversion of either precursor to testosterone was considerably less than in the normal testis. Suggestive evidence is presented for the occurrence of steroid-specific 17α-hydroxylase and 3β-hydroxysteroid dehydrogenase-isomerase systems in canine testicular tissue. The formation of sulphates of pregnenolone and DHA was shown both in normal and in neoplastic tissues and, in addition, the tumour either formed the sulphate of 17α-hydroxypregnenolone or caused the 17α-hydroxylation of pregnenolone sulphate.

STEROID METABOLISM IN VITRO BY AN INTERSTITIAL CELL TUMOUR AND THE ATTACHED PREPUBERTAL TESTIS

1973 ◽  
Vol 59 (3) ◽  
pp. 637-649 ◽  
Author(s):  
GWEN RICHARDS ◽  
A. MUNRO NEVILLE
Keyword(s):  
Metabolic Pathways ◽  
Interstitial Cell ◽  
Reductase Activity ◽  
Testicular Tissue ◽  
Cell Tumour ◽  
Tumour Tissue ◽  
Steroid Metabolism

SUMMARY A virilizing interstitial cell tumour and the attached testicular tissue from a 4-year-old boy were incubated in vitro with [7α-3H]pregnenolone and [4-14C]progesterone, or [4-14C]androstenedione and [7α-3H]5α-dihydrotestosterone. Ring A saturated steroids were produced from 4-ene precursors by the prepubertal testis, but this tissue was unable to convert pregnenolone or progesterone to 17α-hydroxylated C21 steroids, or to C19 steroids. The virilizing interstitial cell tumour metabolized pregnenolone and progesterone to 17α-hydroxyprogesterone, androstenedione and testosterone. In addition, dehydroepiandrosterone was detected as a product of pregnenolone. The tumour lacked 4-ene-5α-steroid reductase activity. 5α-Dihydrotestosterone was metabolized to 5α-androstane-3,17-dione, androsterone, isoandrosterone, 5a-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol in both the normal and tumour tissue. The significance of these metabolic pathways is discussed.

scholarly journals In vivo and in vitro studies on steroid metabolism in a case of primary aldosteronism with multiple lesions of adenoma and nodular hyperplasia.

1982 ◽  
Vol 29 (5) ◽  
pp. 529-540 ◽  
Author(s):  
MASANOBU HONDA ◽  
MAKOTO TSUCHIYA ◽  
HIROO TAMURA ◽  
HIDETO WATANABE ◽  
YOICHI IZUMI ◽  
...  
Keyword(s):  
Primary Aldosteronism ◽  
In Vitro Studies ◽  
Steroid Metabolism ◽  
Multiple Lesions ◽  
Nodular Hyperplasia

In vitro studies on steroid metabolism by gonadal tissues from ambisexual teleosts

1986 ◽  
Vol 62 (2) ◽  
pp. 335-340 ◽  
Author(s):  
Rudolf Reinboth ◽  
Bernd Becker ◽  
Martin Latz
Keyword(s):  
In Vitro Studies ◽  
Steroid Metabolism

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production
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