STEROID METABOLISM OF A SERTOLI CELL TUMOUR OF THE TESTIS OF A DOG WITH FEMINIZATION AND ALOPECIA AND OF THE NORMAL CANINE TESTIS

1967 ◽  
Vol 38 (1) ◽  
pp. 61-NP ◽  
Author(s):  
C. G. PIERREPOINT ◽  
JEAN McI. GALLEY ◽  
K. GRIFFITHS ◽  
J. K. GRANT
Keyword(s):  
Sertoli Cell ◽  
Direct Evidence ◽  
Hydroxysteroid Dehydrogenase ◽  
Testicular Tissue ◽  
Cell Tumour ◽  
Tumour Tissue ◽  
Steroid Metabolism ◽  
Enzyme Systems ◽  
Sertoli Cell Tumour

SUMMARY The metabolism of [7α-3H]pregnenolone and [4-14C]dehydroepiandrosterone (DHA) by a Sertoli cell tumour of the testis from a dog with signs of feminization has been studied in vitro and compared with the metabolism of the normal canine testis. The tumour formed oestrone and oestradiol-17β from DHA thus providing direct evidence for the synthesis of oestrogen by this type of neoplasm. Relative or complete inactivity of several enzyme systems involved in the synthesis of testosterone was found in the tumour tissue, and the conversion of either precursor to testosterone was considerably less than in the normal testis. Suggestive evidence is presented for the occurrence of steroid-specific 17α-hydroxylase and 3β-hydroxysteroid dehydrogenase-isomerase systems in canine testicular tissue. The formation of sulphates of pregnenolone and DHA was shown both in normal and in neoplastic tissues and, in addition, the tumour either formed the sulphate of 17α-hydroxypregnenolone or caused the 17α-hydroxylation of pregnenolone sulphate.

STEROID METABOLISM IN VITRO BY AN INTERSTITIAL CELL TUMOUR AND THE ATTACHED PREPUBERTAL TESTIS

1973 ◽  
Vol 59 (3) ◽  
pp. 637-649 ◽  
Author(s):  
GWEN RICHARDS ◽  
A. MUNRO NEVILLE
Keyword(s):  
Metabolic Pathways ◽  
Interstitial Cell ◽  
Reductase Activity ◽  
Testicular Tissue ◽  
Cell Tumour ◽  
Tumour Tissue ◽  
Steroid Metabolism

SUMMARY A virilizing interstitial cell tumour and the attached testicular tissue from a 4-year-old boy were incubated in vitro with [7α-3H]pregnenolone and [4-14C]progesterone, or [4-14C]androstenedione and [7α-3H]5α-dihydrotestosterone. Ring A saturated steroids were produced from 4-ene precursors by the prepubertal testis, but this tissue was unable to convert pregnenolone or progesterone to 17α-hydroxylated C21 steroids, or to C19 steroids. The virilizing interstitial cell tumour metabolized pregnenolone and progesterone to 17α-hydroxyprogesterone, androstenedione and testosterone. In addition, dehydroepiandrosterone was detected as a product of pregnenolone. The tumour lacked 4-ene-5α-steroid reductase activity. 5α-Dihydrotestosterone was metabolized to 5α-androstane-3,17-dione, androsterone, isoandrosterone, 5a-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol in both the normal and tumour tissue. The significance of these metabolic pathways is discussed.

Germline mutation in the STK11 gene in a girl with an ovarian Sertoli cell tumour

2006 ◽  
Vol 166 (10) ◽  
pp. 1083-1085 ◽  
Author(s):  
Guy Massa ◽  
Nele Roggen ◽  
Marleen Renard ◽  
Johan J. P. Gille
Keyword(s):  
Sertoli Cell ◽  
Germline Mutation ◽  
Cell Tumour ◽  
Stk11 Gene ◽  
Sertoli Cell Tumour

scholarly journals MALIGNANT SERTOLI CELL TUMOUR: AN UNCOMMON TESTICULAR TUMOUR

2006 ◽  
Vol 9 (2) ◽  
pp. 18-21
Author(s):  
Sia SF ◽  
Dublin N ◽  
Nurul B ◽  
Wong KT
Keyword(s):  
Sertoli Cell ◽  
Cell Tumour ◽  
Testicular Tumour ◽  
Sertoli Cell Tumour

Large-Cell Calcifying Sertoli Cell Tumour of the Testis Detected at Screening of a Family with Carney Syndrome

2005 ◽  
Vol 75 (4) ◽  
pp. 365-367 ◽  
Author(s):  
S.N. Jayasena ◽  
J.T.N. Ariyasinghe ◽  
D.M.R. Gunawardena ◽  
S.A.S. Gunawardena ◽  
M.V.C. de Silva
Keyword(s):  
Sertoli Cell ◽  
Large Cell ◽  
Cell Tumour ◽  
Sertoli Cell Tumour ◽  
Carney Syndrome

17β-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN HUMAN TESTES

1967 ◽  
Vol 56 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Edgar J. Schoen
Keyword(s):  
Dehydrogenase Activity ◽  
Conversion Rate ◽  
Prostatic Carcinoma ◽  
Hydroxysteroid Dehydrogenase ◽  
Testicular Tissue

ABSTRACT In vitro 17β-hydroxysteroid dehydrogenase activity in testicular tissue from 7 men subjected to orchiectomy for prostatic carcinoma was measured by the conversion rate of androstenedione to testosterone. Prior to orchiectomy, 3 of the patients were untreated: 3 had received stilboestrol. 5 mg daily for one month; 1 had received stilboestrol, 2 mg daily for one month. There was evidence that stilboestrol in a dosage of 5 mg daily for one month prior to orchiectomy led to suppression of testosterone formation from androstenedione. 17β-Hydroxysteroid dehydrogenase activity could be demonstrated in small quantities of testicular homogenate, and thus offers an additional technique for assessing testicular androgenic function in man.

A COMPARATIVE STUDY OF THE BIOSYNTHESIS OF TESTOSTERONE BY HUMAN TESTES AND A VIRILIZING INTERSTITIAL CELL TUMOUR

1967 ◽  
Vol 56 (4) ◽  
pp. 726-736 ◽  
Author(s):  
D. C. Sharma ◽  
E. A. Racz ◽  
R. I. Dorfman ◽  
E. J. Schoen
Keyword(s):  
Interstitial Cell ◽  
Testicular Tissue ◽  
Cell Tumour ◽  
Isotopic Dilution ◽  
Testicular Tumour ◽  
Early Puberty ◽  
Laboratory Findings ◽  
Major Reaction ◽  
The Right

ABSTRACT A comparative in vitro study of an interstitial cell tumour of the testis from a virilized patient and »normal« testes from another male has been made by incubating homogenates of these tissues with radioactive progesterone, 17α-hydroxyprogesterone, testosterone and androst-4-ene-3,17-dione-4-14C. The patient with the testicular tumour was a 14-year old boy whose clinical studies, reported in detail elsewhere, revealed a history of early puberty and laboratory findings of markedly elevated levels of urinary total 17-ketosteroids and pregnanetriol. These urinary values fell to normal following removal of the right testicular tumour, which was interpreted as being a benign interstitial cell tumour on histologic examination. The radioactive metabolites from the testicular tissue of both subjects were separated, purified and identified by partition column chromatography in various systems with authentic standards, formation of derivatives and isotopic dilution analysis. The interstitial cell tumour converted a significant amount of the substrate progesterone into 16α-hydroyprogesterone, besides testosterone and androst-4-ene-3,17-dione; reduction at C20 was also a major reaction when either progesterone or 17α-hydroxyprogesterone was the substrate. On a comparative basis the activity of C17-20 desmolase was about five times more in the tumour as compared to the testicular tissue.

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