Adenyl cyclase activity in isolated fat cells from cold exposed rats

Life Sciences ◽  
1969 ◽  
Vol 8 (24) ◽  
pp. 1353-1358 ◽  
Author(s):  
D.G. Therriault ◽  
J.F. Morningstar ◽  
V.G. Winters
Biochemistry ◽  
1969 ◽  
Vol 8 (7) ◽  
pp. 3092-3099 ◽  
Author(s):  
Martha Vaughan ◽  
Ferid Murad

Reproduction ◽  
1974 ◽  
Vol 36 (2) ◽  
pp. 445-446
Author(s):  
C. Nugent ◽  
A Lopata ◽  
M. Gould

1972 ◽  
Vol 20 (11) ◽  
pp. 873-879 ◽  
Author(s):  
S. L. HOWELL ◽  
MARGARET WHITFIELD

A cytochemical method has been used to investigate the localization of adenyl cyclase activity in A and B cells of isolated rat islets of Langerhans. Adenosine triphosphate was initially utilized as substrate, the pyrophosphate liberated being precipitated by lead ions at its site of production. The specificity of the method was increased by the use of adenylyl-imidodiphosphate as an alternative substrate; this adenosine triphosphate analogue was not hydrolyzed by adenosine triphosphatase but provided an effective substrate for adenyl cyclase. Adenyl cyclase activity, which was found to retain its glucagon and fluoride sensitivity in glutaraldehyde-fixed tissue, was found exclusively and almost uniformly in the plasma membranes of A and B cells. Storage granule membrane, incorporated into the plasma membrane during secretion of the granule content by exocytosis, appeared to be devoid of adenyl cyclase activity.


Circulation ◽  
1971 ◽  
Vol 44 (4) ◽  
pp. 638-648 ◽  
Author(s):  
ROBERT E. GOLDSTEIN ◽  
C. LYNN SKELTON ◽  
GERALD S. LEVEY ◽  
D. LUKE GLANCY ◽  
G. DAVID BEISER ◽  
...  

1976 ◽  
Vol 256 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Carla Cipriani ◽  
G. Moretti ◽  
E. Rampini ◽  
Carla Divano

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