Effect of aging on the adenyl cyclase and phosphodiesterase activity of isolated fat cells of rat

1970 ◽  
Vol 208 (2) ◽  
pp. 304-309 ◽  
Author(s):  
J. Forn ◽  
P.S. Schonhofer ◽  
I.F. Skidmore ◽  
G. Krishna
1970 ◽  
Vol 120 (1) ◽  
pp. 187-193 ◽  
Author(s):  
E. G. Loten ◽  
J. G. T. Sneyd

1. 3′:5′-Cyclic nucleotide phosphodiesterase activity was measured in homogenates prepared from epididymal fat-pads and isolated fat-cells incubated in the absence and presence of insulin. 2. Homogenates of insulin-treated tissues showed an increase in phosphodiesterase activity compared with controls. No effect of insulin was observed when the hormone was added directly to homogenates. 3. There was kinetic evidence for the presence of two 3′:5′-cyclic nucleotide phosphodiesterases in adipose tissue. Insulin raised the maximal velocity of the low-Km enzyme and lowered the Km of the higher-Km enzyme. 4. It is suggested that the effect of insulin on adipose tissue phosphodiesterase accounts for the ability of this hormone to lower cyclic-AMP concentration in the tissue.


1972 ◽  
Vol 273 (3) ◽  
pp. 267-282 ◽  
Author(s):  
P. S. Sch�nh�fer ◽  
I. F. Skidmore ◽  
M. I. Paul ◽  
B. R. Ditzion ◽  
G. L. Pauk ◽  
...  

Life Sciences ◽  
1969 ◽  
Vol 8 (24) ◽  
pp. 1353-1358 ◽  
Author(s):  
D.G. Therriault ◽  
J.F. Morningstar ◽  
V.G. Winters

1970 ◽  
Vol 245 (4) ◽  
pp. 718-722
Author(s):  
Martin Rodbell ◽  
Lutz Birnbaumer ◽  
Stephen L. Pohl
Keyword(s):  

1969 ◽  
Vol 244 (19) ◽  
pp. 5181-5188
Author(s):  
T. Minemura ◽  
Oscar B. Crofford

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