Serological diagnosis of neurocysticercosis: evaluation of ELISA tests using cyst fluid and other components of Taenia solium cysticerci as antigens

1988 ◽  
Vol 82 (2) ◽  
pp. 295-299 ◽  
Author(s):  
G.G. Baily ◽  
P.R. Mason ◽  
F.E.J. Trijssenar ◽  
N.F. Lyons
Keyword(s):  
Taenia Solium ◽  
Cyst Fluid ◽  
Serological Diagnosis

scholarly journals Serological Diagnosis of Human Cysticercosis by Use of Recombinant Antigens from Taenia solium Cysticerci

1999 ◽  
Vol 6 (4) ◽  
pp. 479-482 ◽  
Author(s):  
Kerstin Hubert ◽  
Abel Andriantsimahavandy ◽  
Alain Michault ◽  
Matthias Frosch ◽  
Fritz A. Mühlschlegel
Keyword(s):  
Immunosorbent Assay ◽  
Fusion Proteins ◽  
Taenia Solium ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cdna Expression Library ◽  
Serum Samples ◽  
Expression Library ◽  
Recombinant Antigens ◽  
Cdna Expression

ABSTRACT A Taenia solium metacestode cDNA expression library in the lambda ZAPII vector was screened with pooled sera from patients with neurocysticercosis. Sixty primary clones were identified and shown to belong to two classes. The clones NC-3 and NC-9 did not reveal any significant homologies to sequences deposited in the databases and were further characterized. Both recombinant antigens were expressed as glutathione S-transferase fusion proteins and applied for serological diagnosis of human cysticercosis. An enzyme-linked immunosorbent assay was established and evaluated with 27 serum samples of La Réunion and Madagascar patients with cysticercosis. Diagnosis in these patients was established with radiological and serological procedures. For antigen NC-3 a sensitivity of 96.3% and a specificity of 91.5% for the serodiagnosis were achieved. In contrast, the sensitivity of antigen NC-9 was only 33.3%.

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