Isolation of a 14kDa antigen from Taenia solium cyst fluid by HPLC and its evaluation in enzyme linked immunosorbent assay for diagnosis of porcine cysticercosis

2007 ◽  
Vol 82 (3) ◽  
pp. 370-376 ◽  
Author(s):  
E. Assana ◽  
K. Kanobana ◽  
C.B. Tume ◽  
P.A. Zoli ◽  
Nguekam ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Cyst Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Porcine Cysticercosis

scholarly journals Evidence for Transmission of Taenia solium Taeniasis/Cysticercosis in a Rural Area of Northern Rwanda

2021 ◽  
Vol 8 ◽  
Author(s):  
Lucrecia Acosta Soto ◽  
Lucy Anne Parker ◽  
María José Irisarri-Gutiérrez ◽  
Javier Arturo Bustos ◽  
Yesenia Castillo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Low Income ◽  
Taenia Solium ◽  
Parasitic Infection ◽  
Developed Countries ◽  
Cross Sectional Study ◽  
Low Income Countries ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Hygiene Measures

Cysticercosis is a parasitic infection caused by the metacestode larval stage (cysticercus) of Taenia solium. In humans, cysticercosis may infect the central nervous system and cause neurocysticercosis, which is responsible for over 50,000 deaths per year worldwide and is the major cause of preventable epilepsy cases, especially in low-income countries. Cysticercosis infection is endemic in many less developed countries where poor hygiene conditions and free-range pig management favor their transmission. A cross-sectional study was conducted in 680 children from a rural primary school in Gakenke district (Northern province of Rwanda). Stool samples were collected from participants and analyzed using the Kato-Katz method (KK), formol-ether concentration (FEC), and/or copro-antigen enzyme-linked immunosorbent assay (CoAg-ELISA) to detect taeniasis. Blood samples were collected and analyzed using enzyme-linked immunoelectrotransfer blot (EITB) and antigen enzyme-linked immunosorbent assay (Ag-ELISA) to detect human cysticercosis. The overall proportion of taeniasis positivity was 0.3% (2/680), and both cases were also confirmed by CoAg-ELISA. A total of 13.3% (76/572) of the children studied were positive to cysticercosis (T. solium-specific serum antibodies detected by EITB), of whom 38.0% (27/71) had viable cysticercus (T. solium antigens by Ag-ELISA). This study provides evidence of the highest cysticercosis prevalence reported in Rwanda in children to date. Systematic investigations into porcine and human cysticercosis as well as health education and hygiene measures for T. solium control are needed in Gakenke district.

scholarly journals Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

2002 ◽  
Vol 44 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Lúcia M. BRAGAZZA ◽  
Adelaide J. VAZ ◽  
Afonso D.C. PASSOS ◽  
Osvaldo M. TAKAYANAGUI ◽  
Paulo M. NAKAMURA ◽  
...  
Keyword(s):  
Public Health ◽  
Immunosorbent Assay ◽  
Taenia Solium ◽  
High Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Taenia Crassiceps ◽  
Municipal District ◽  
The Impact

Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeirão Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1%.

scholarly journals Co-infection of pigs with Taenia solium cysticercosis and gastrointestinal parasites in Eastern and Western Uganda

2021 ◽  
Author(s):  
Nicholas Ngwili ◽  
Lian Thomas ◽  
Samuel Githigia ◽  
Dishon Muloi ◽  
Karen Marshall ◽  
...  
Keyword(s):  
Cost Benefit Analysis ◽  
Taenia Solium ◽  
Cost Benefit ◽  
Gastrointestinal Parasites ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infection Status ◽  
Gastrointestinal Helminths ◽  
Porcine Cysticercosis ◽  
Circulating Antigen ◽  
Control And Management

AbstractA study was carried out in Kamuli and Hoima districts in Eastern and Western regions of Uganda to determine the Taenia solium porcine cysticercosis (PCC) and gastrointestinal (GI) parasites co-infection status in pigs. One hundred sixty-one households were selected randomly and visited between November and December 2019. A household questionnaire was administered, and faecal and blood samples were collected from at least one pig older than 3 months per household. A blood sample was obtained from a jugular venipuncture, and a rectal faecal sample was obtained. Taenia spp. circulating antigen levels in the sample sera were tested using a commercial enzyme-linked immunosorbent assay kit, apDia™ cysticercosis Ag ELISA. The modified McMaster technique was used to identify and quantify the GI parasites. The apparent animal-level seroprevalence for PCC was 4.8% (95% CI 2.7–7.1) and differed across the two districts (p = 0.018). At the pig herd level, the prevalence was 9.7% (95% CI 5.5–14.4). The prevalence of the different nematode eggs and coccidian oocysts in the two districts was as follows: strongyles 79.0% (95% CI 74.3–83.6), coccidia 73.3% (95% CI 68.3–78.6), Trichuris spp. 7.4% (95% CI 4.9–10.6), Strongyloides ransomi 2.1 (95% CI 0.7–3.5) and Ascaris spp. 4.9 (95% CI 2.8–7.4). Overall, across the two districts, the arithmetic mean for the oocysts per gram (OPG) for coccidia was 2042.2 ± 5776.1, and eggs per gram (EPG) were the highest in strongyles 616.1 ± 991. Overall, 57.4% of the porcine cysticercosis seropositive pigs were also positive for at least one of the gastrointestinal helminths which included strongyles, Strongyloides ransomi, Trichuris spp. and Ascaris spp. The co-infection status of pigs with both PCC and GI parasites demonstrated by this study can provide an incentive for integrating the control and management of both parasites with oxfendazole. Further studies are required to understand the feasibility of using oxfendazole including cost–benefit analysis and the acceptability by local stakeholders for the control of T. solium cysticercosis and gastrointestinal parasites in pigs.

Enzyme-Linked Immunosorbent Assay (Elisa) for the Detection of Antibody to Cysticerci of Taenia Solium

1982 ◽  
Vol 31 (2) ◽  
pp. 364-369 ◽  
Author(s):  
Arwin R. Diwan ◽  
Millicent Coker-Vann ◽  
Paul Brown ◽  
D. B. Subianto ◽  
D. Carleton Gajdusek ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

Discrimination between active and inactive neurocysticercosis by metacestode excretory/secretory antigens of Taenia solium in an enzyme-linked immunosorbent assay.

2002 ◽  
Vol 66 (6) ◽  
pp. 777-781 ◽  
Author(s):  
José L Molinari ◽  
Julio Sotelo ◽  
Patricia Tato ◽  
Yazmin de la Garza ◽  
Esperanza García-Mendoza ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Seroprevalensi Sistiserkosis pada Babi di Wilayah Wamena, Papua

2020 ◽  
pp. 67
Author(s):  
Alvionita Alexther Lingga ◽  
Ida Bagus Ngurah Swacita ◽  
I Ketut Suada
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

Sistiserksosis merupakan penyakit zoonosis disebabkan oleh larva cacing Taenia solium yang berdampak serius pada kesehatan hewan maupun manusia. Papua merupakan salah satu daerah endemis sistiserkosis di Indonesia. Penelitian ini bertujuan untuk mengetahui seroprevalensi sistiserkosis pada babi di wilayah Wamena, Papua. Sebanyak seratus sebelas sampel serum babi yang disampling dari wilayah Wamena kemudian diuji dengan Enzyme Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan bahwa seroprevalensi sistiserkosis pada sampel babi tersebut sebanyak 39,64% (44/111). Dapat disimpulkan bahwa seroprevalensi sistiserkosis pada sampel babi dari Wilayah Wamena, Papua sangat tinggi, dengan demikian maka diperlukan program untuk memberantas penyakit ini oleh instansi terkait di Wilayah Wamena, Papua

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