Effect of hexadimethrine bromide on the conversion of 4-14C-progesterone into aldosterone and corticosterone by rat adrenals

SummaryThe cold promoted activation of factor VII occurs in parallel with an activation of a plasma arginine esterase, and, on inhibition of the cold activation of factor VII, the esterase activation also decreased. The inhibitor pattern supported our theory that the arginine esterase that is activated in the cold activation of factor VII is plasma kallikrein.The cold activation of factor VII was completely inhibited with soya bean trypsin inhibitor in doses that did not interfere with the contact activation. On the other hand, inhibition of the contact activation with hexadimethrine bromide did not interfere with the cold activation of factor VII except when this was kaolin induced. Contact and cold activation therefore appear to represent two different pathways for the activation of factor VII. The cold activation reaction is probably mediated by the activation of plasma prekallikrein, and inhibition of the plasma kallikrein activity correlates with the inhibition of the cold promoted activation of factor VII.

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EFFECT OF HYPOPHYSECTOMY ON CHANGES CAUSED BY HEXADIMETHRINE BROMIDE

Acta Endocrinologica ◽

10.1530/acta.0.056s045 ◽

1967 ◽

Vol 56 (1_Suppl) ◽

pp. S45

Author(s):

F. A. László ◽

I. Szijj ◽

J. Kocsis ◽

K. Kovács

Keyword(s):

Hexadimethrine Bromide

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Faculty Opinions recommendation of Corticotropin-releasing factor (CRF) and the urocortins differentially regulate catecholamine secretion in human and rat adrenals, in a CRF receptor type-specific manner.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1057989.509942 ◽

2007 ◽

Author(s):

Yvette Taché

Keyword(s):

Corticotropin Releasing Factor ◽

Receptor Type ◽

Catecholamine Secretion ◽

Specific Manner ◽

Rat Adrenals

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DNA hypermethylation of acetoacetyl-CoA synthetase contributes to inhibited cholesterol supply and steroidogenesis in fetal rat adrenals under prenatal nicotine exposure

Toxicology ◽

10.1016/j.tox.2016.01.002 ◽

2016 ◽

Vol 340 ◽

pp. 43-52 ◽

Cited By ~ 5

Author(s):

Dong-Mei Wu ◽

Zheng He ◽

Ting Chen ◽

Yang Liu ◽

Liang-Peng Ma ◽

...

Keyword(s):

Nicotine Exposure ◽

Dna Hypermethylation ◽

Prenatal Nicotine Exposure ◽

Fetal Rat ◽

Rat Adrenals ◽

Prenatal Nicotine

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The properties of adrenal zona glomerulosa cells after purification by gravitational sedimentation

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1974.0025 ◽

1974 ◽

Vol 185 (1081) ◽

pp. 375-407 ◽

Cited By ~ 39

Keyword(s):

Angiotensin Ii ◽

Cyclic Amp ◽

Microscopic Examination ◽

Zona Glomerulosa ◽

Maximal Response ◽

Zona Fasciculata ◽

Gravitational Sedimentation ◽

Rat Adrenals ◽

Glomerulosa Cells ◽

Zona Glomerulosa Cells

The densities of latex spheres and biological cells can be reliably determined from their sedimentation rate in an albumin gradient under unit gravitational force. The densities of zona glomerulosa and fasciculata cells of rat adrenals were found to be 1.072 ± 0.004 and 1.040 ± 0.001 respectively. Purified zona glomerulosa cells of rat adrenals can be prepared by gravitational sedimentation of dispersed cells from capsule strippings of the gland, which originally contain 3 to10% zona fasciculata contamination. Electron and phase microscopic examination of the sedimented glomerulosa cells and their steroidogenic response to ACTH and cyclic AMP indicate that they are reasonably free of contamination from zona fasciculata cells. Electron microscopic examination of the purified glomerulosa cells indicates that most of them are reasonably normal in structure. Their basal production of corticosterone is decreased after sedimentation. However, their maximal response of corticosterone output to serotonin and potassium and their response to all potassium concentrations is not significantly altered, indicating normal function for the cells producing steroids. Their maximal responses to ACTH, valine angiotensin II and cyclic AMP are decreased, but, at the doses used, steroidogenesis by the zona fasciculata contamination in the unfractionated preparation would be stimulated by these substances. Purified zona glomerulosa cells have about the same maximal response of corticosterone output (about twofold) to potassium, valine and isoleucine angiotensin II, serotonin and ACTH. The maximal response of the purified zona glomerulosa cells to cyclic AMP is similar to that elicited by valine and isoleucine angiotensin II, potassium, serotonin or ACTH. This indicates that if these stimuli act by increasing cyclic AMP output, then the maximal response of corticosterone output (about twofold) is defined by the limited response of the biosynthetic pathways to cyclic AMP.

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