Immunocytochemical localization of FMRFamide in the central nervous system and the kidney of Helisoma duryi (Mollusca): Its possible antidiuretic role

1992 ◽  
Vol 24 (2) ◽  
pp. 179-189 ◽  
Author(s):  
A.S.M. Saleuddin ◽  
Hamid R. Khan ◽  
Mary-Lou Ashton ◽  
Beŕnadette Griffond
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Immunocytochemical Localization ◽  
Helisoma Duryi ◽  
The Central Nervous System

scholarly journals Acrolein: a fixative for immunocytochemical localization of peptides in the central nervous system.

1983 ◽  
Vol 31 (1) ◽  
pp. 62-68 ◽  
Author(s):  
J C King ◽  
R M Lechan ◽  
G Kugel ◽  
E L Anthony
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Tyrosine Hydroxylase ◽  
Adrenocorticotropic Hormone ◽  
Immunocytochemical Localization ◽  
Vascular Perfusion ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Morphological Detail ◽  
The Central Nervous System

Acrolein was examined as an alternative fixative to formaldehyde for immunocytochemical localization of neuropeptides in the rat brain. A brief (5 min) vascular perfusion with a 5% acrolein solution allowed the identification of thyrotropin-releasing hormone (TRH), vasoactive intestinal peptide (VIP), somatostatin (SRIF), neurotensin (NT), methionine enkephalin (Menk), adrenocorticotropic hormone (ACTH), tyrosine hydroxylase (TH), and luteinizing hormone-releasing hormone (LHRH) in fibers and perikarya within the central nervous system of the rat using the peroxidase-antiperoxidase (PAP) technique. Acrolein appears to be particularly valuable for immunocytochemistry, as it 1) stabilizes heterogeneous peptides and proteins rapidly and effectively, 2) retains antigenicity, and 3) preserves morphological detail.

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