Monoclonal antibodies to VRV-PL-VIIIa, a basic multitoxic phospholipase A2 from Vipera russelli venom

Toxicon ◽  
1994 ◽  
Vol 32 (8) ◽  
pp. 955-964 ◽  
Author(s):  
T.Veerabasappa Gowda ◽  
John L. Middlebrook
Keyword(s):  
Monoclonal Antibodies ◽  
Phospholipase A2 ◽  
Vipera Russelli

scholarly journals Monoclonal antibodies specific to a Ca2+-bound form of lipocortin I distinguish its Ca2+-dependent phospholipid-binding ability from its ability to inhibit phospholipase A2

1990 ◽  
Vol 269 (3) ◽  
pp. 709-715 ◽  
Author(s):  
H Hayashi ◽  
M K Owada ◽  
S Sonobe ◽  
K Domae ◽  
T Yamanouchi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Phospholipase A2 ◽  
Inhibitory Activity ◽  
Biological Effects ◽  
Free Form ◽  
E Coli ◽  
Phospholipid Binding ◽  
Ef Hand

Lipocortin I, a Ca2(+)-and phospholipid-binding protein without EF-hand structures, has many biological effects in vitro. Its actual role in vivo, however is unknown. We obtained and characterized five monoclonal antibodies to lipocortin I. Two of these monoclonal antibodies (L2 and L4-MAbs) reacted with the Ca(+)-bound form of lipocortin I, but not with the Ca2(+)-free form, both in vivo and in vitro. Lipocortin I required greater than or equal to 10 microM-Ca2+ to bind the two antibodies, and this Ca2+ requirement was not affected by phosphatidylserine. L2-MAb abolished the phospholipase A2 inhibitory activity of lipocortin I and inhibited its binding to Escherichia coli membranes and to phosphatidylserine in vitro. L4-MAb abolished the phospholipase A2 inhibitory activity of lipocortin I, but did not affect its binding to E. coli membranes or to phosphatidylserine. These findings indicated that the inhibition of phospholipase A2 by lipocortin I was not simply due to removal or capping of the substrates in E. coli membranes. Furthermore, an immunofluorescence study using L2-MAb showed the actual existence of Ca2(+)-bound form of lipocortin I in vivo.

Immunochemical properties of Naja naja atra (Taiwan cobra) phospholipase A2 using polyclonal and monoclonal antibodies

Toxicon ◽  
1992 ◽  
Vol 30 (2) ◽  
pp. 151-159 ◽  
Author(s):  
C.C. Yang ◽  
L.S. Chang ◽  
P.L. Ong ◽  
T.H. Tung
Keyword(s):  
Monoclonal Antibodies ◽  
Phospholipase A2 ◽  
Naja Naja ◽  
Naja Atra

Solution-phase binding of monoclonal antibodies to bee venom phospholipase A2

1994 ◽  
Vol 177 (1-2) ◽  
pp. 35-42 ◽  
Author(s):  
Elizabeth A. Myatt ◽  
Fred J. Stevens ◽  
Chris Benjamin
Keyword(s):  
Monoclonal Antibodies ◽  
Phospholipase A2 ◽  
Solution Phase ◽  
Bee Venom

Immunochemical analysis of a snake venom phospholipase A2 neurotoxin, crotoxin, with monoclonal antibodies

1992 ◽  
Vol 29 (7-8) ◽  
pp. 871-882 ◽  
Author(s):  
V. Choumet ◽  
G. Faure ◽  
A. Robbe-Vincent ◽  
B. Saliou ◽  
J.C. Mazié ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Phospholipase A2 ◽  
Snake Venom ◽  
Immunochemical Analysis ◽  
Snake Venom Phospholipase
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