A murine cell line producing HTLV-I pseudotype virions carrying a selectable marker gene

Virology ◽  
1991 ◽  
Vol 180 (1) ◽  
pp. 420-424 ◽  
Author(s):  
Richard G. Vile ◽  
Thomas F. Schulz ◽  
Olivier F. Danos ◽  
Mary K.L. Collins ◽  
Robin A. Weiss
Keyword(s):  
Cell Line ◽  
Selectable Marker ◽  
Marker Gene ◽  
Selectable Marker Gene ◽  
Murine Cell ◽  
Murine Cell Line

235 PRODUCTION OF PORCINE KNOCK-IN FIBROBLASTS EXPRESSING HUMAN DECAY-ACCELERATING FACTOR on α-1,3-GALACTOSYLTRANSFERASE (GGTA1) GENE LOCUS

2012 ◽  
Vol 24 (1) ◽  
pp. 230
Author(s):  
J. W. Kim ◽  
S. M. Lee ◽  
J. W. Ju ◽  
M. J. Kang
Keyword(s):  
Somatic Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Selectable Marker ◽  
Marker Gene ◽  
Regulatory Protein ◽  
Foreign Gene ◽  
Organ Donors ◽  
Selectable Marker Gene ◽  
Decay Accelerating Factor

Galactose-α1,3-galactose (α1,3-gal) epitope is synthesised at a high concentration on the surface of pig cells by α1,3-galactosyltransferase gene (GGTA1). The α1,3-gal is responsible for hyperacute rejection in pig-to-human xenotransplantation. The generation of transgenic pigs as organ donors for humans is necessary to eliminate the GGTA1 gene that synthesise Galα(1,3)Gal. Knock-in system refers to the integration a foreign gene at a specific target, disrupting the target gene by inserting the transgene. Human decay-accelerating factor (hDAF) is a cell surface regulatory protein that serves as complement inhibitor to protect self cells from complement attack. The generation of GGTA1 knockout pigs expressing DAF is necessary to use as organ donors for humans. In this study, we established cell lines of GGTA1 knockout expressing DAF from pig ear fibroblasts to apply somatic cell nuclear transfer. A GGTA1-DAF knock-in vector for positive-negative selection (PNS) consists of the neomycin resistance gene (neo) as a positive selectable marker gene and the diphtheria toxin A fragment (DT-A) gene as a negative selectable marker gene. The DAF gene was subcloned in the GGTA1 gene exon 4 and the endogenous start codon was replaced by that of DAF. The ear fibroblast cell lines were isolated from Chicago miniature pig and used for transfection. Linearized targeting vector DNA was transfected into these cells by electroporation. After selection of G-418 during 11 days, we analysed 242 colonies using PCR and long PCR. We isolated 2 positive colonies for GGTA1 gene disruption. To confirm expression of the hDAF and GGTA1 genes, we isolated total RNA from normal pig cells and the knock-in somatic cell line. A cDNA synthesised using reverse transcriptase progressed to RT-PCR by a pair of DAF-specific primers and α1,3-GT specific primer, respectively. Expression of DAF was detected in DAF knock-in somatic cells, butnot in normal pig cell. Expression of GGTA1 gene was decreased in the knock-in somatic cell line compared with normal pig cell. These cell lines may be used production of α1,3-GT knockout and DAF expression pig for xenotransplantation.

A plant selectable marker gene based on the detoxification of the herbicide dalapon

1992 ◽  
Vol 11 (12) ◽  
Author(s):  
Vicky Buchanan-Wollaston ◽  
Andrea Snape ◽  
Frank Cannon
Keyword(s):  
Selectable Marker ◽  
Marker Gene ◽  
Selectable Marker Gene

scholarly journals Manipulating genome of diploid potato inbred line Solanumchacoense M6 using selectable marker gene

2020 ◽  
Vol 44 (4) ◽  
pp. 399-407
Author(s):  
Sarbesh Das DANGOL ◽  
İlknur YEL ◽  
Mehmet Emin ÇALIŞKAN ◽  
Allah BAKHSH
Keyword(s):  
Inbred Line ◽  
Selectable Marker ◽  
Marker Gene ◽  
Diploid Potato ◽  
Selectable Marker Gene

Human interleukin 2 (IL 2) receptor β chain allows transduction of IL 2-induced proliferation signal(s) in a murine cell line

1989 ◽  
Vol 19 (12) ◽  
pp. 2375-2378 ◽  
Author(s):  
Takeshi Doi ◽  
Masanori Hatakeyama ◽  
Seijiro Minamoto ◽  
Takeshi Kono ◽  
Hisashi Mori ◽  
...  
Keyword(s):  
Cell Line ◽  
Interleukin 2 ◽  
Murine Cell ◽  
Murine Cell Line ◽  
Β Chain

scholarly journals Retroviral insertional mutagenesis of a target allele in a heterozygous murine cell line.

1985 ◽  
Vol 82 (19) ◽  
pp. 6600-6604 ◽  
Author(s):  
W. Frankel ◽  
T. A. Potter ◽  
N. Rosenberg ◽  
J. Lenz ◽  
T. V. Rajan
Keyword(s):  
Cell Line ◽  
Insertional Mutagenesis ◽  
Murine Cell ◽  
Murine Cell Line
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