Bright field electron microscopy of biological specimens—VI Signal-to-noise ratio in specimens prepared on amorphous carbon and graphite crystal supports

Micron (1969) ◽  
1976 ◽  
Vol 7 (2) ◽  
pp. 157-170 ◽  
Author(s):  
B.V. Johansen
Author(s):  
W. Kunath ◽  
K. Weiss ◽  
E. Zeitler

Bright-field images taken with axial illumination show spurious high contrast patterns which obscure details smaller than 15 ° Hollow-cone illumination (HCI), however, reduces this disturbing granulation by statistical superposition and thus improves the signal-to-noise ratio. In this presentation we report on experiments aimed at selecting the proper amount of tilt and defocus for improvement of the signal-to-noise ratio by means of direct observation of the electron images on a TV monitor.Hollow-cone illumination is implemented in our microscope (single field condenser objective, Cs = .5 mm) by an electronic system which rotates the tilted beam about the optic axis. At low rates of revolution (one turn per second or so) a circular motion of the usual granulation in the image of a carbon support film can be observed on the TV monitor. The size of the granular structures and the radius of their orbits depend on both the conical tilt and defocus.


2015 ◽  
Vol 21 (S3) ◽  
pp. 963-964
Author(s):  
Peng Gao ◽  
Ryo Ishikawa ◽  
Eita Tochigi ◽  
Akihito Kumamoto ◽  
Naoya Shibata ◽  
...  

2011 ◽  
Vol 10 (4) ◽  
pp. 278-281 ◽  
Author(s):  
Ryo Ishikawa ◽  
Eiji Okunishi ◽  
Hidetaka Sawada ◽  
Yukihito Kondo ◽  
Fumio Hosokawa ◽  
...  

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