[23] Screening of complementary DNA library using radiolabeled antigen

1993 ◽  
pp. 325-335
Author(s):  
Julie Chao ◽  
Karl X. Chai ◽  
Lee Chao
Keyword(s):  
Complementary Dna ◽  
Dna Library

Complementary DNA library construction and expressed sequence tag analysis of an Arctic moss, Aulacomnium turgidum

Polar Biology ◽  
2009 ◽  
Vol 33 (5) ◽  
pp. 617-626 ◽  
Author(s):  
Shenghao Liu ◽  
Hyoungseok Lee ◽  
Pil-Sung Kang ◽  
Xiaohang Huang ◽  
Joung Han Yim ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Complementary Dna ◽  
Library Construction ◽  
Dna Library ◽  
Expressed Sequence Tag Analysis ◽  
Expressed Sequence ◽  
Aulacomnium Turgidum

scholarly journals Strandedness during cDNA synthesis, the stranded parameter in htseq-count and analysis of RNA-Seq data

2020 ◽  
Vol 19 (5-6) ◽  
pp. 339-342 ◽  
Author(s):  
Krishna A Srinivasan ◽  
Suman K Virdee ◽  
Andrew G McArthur
Keyword(s):  
Transcript Abundance ◽  
Rna Seq ◽  
Cdna Synthesis ◽  
Complementary Dna ◽  
Library Construction ◽  
Dna Library ◽  
Subsequent Determination ◽  
Analysis Of Results ◽  
Reference Genomes

Abstract RNA sequencing (RNA-Seq) is a complicated protocol, both in the laboratory in generation of data and at the computer in analysis of results. Several decisions during RNA-Seq library construction have important implications for analysis, most notably strandedness during complementary DNA library construction. Here, we clarify bioinformatic decisions related to strandedness in both alignment of DNA sequencing reads to reference genomes and subsequent determination of transcript abundance.

Sequence heterogeneity of murine complementary DNA clones related to the C4 and C4-Slp isoforms of the fourth complement component

1984 ◽  
Vol 306 (1129) ◽  
pp. 389-394 ◽  
Keyword(s):  
Restriction Site ◽  
Nucleotide Sequencing ◽  
Nucleotide Substitutions ◽  
Complementary Dna ◽  
Dna Library ◽  
Genes Encoding ◽  
Pair Sequence ◽  
The One ◽  
Carboxy Terminal ◽  
A Site

Two classes of mRNA encoding the murine C4 protein were identified by sequence analysis of clones isolated from a liver complementary DNA library. The divergence found within a 357 base pair sequence available for comparison is limited to five nucleotide replacements located in the region corresponding to the carboxy-terminal end of the C4d peptide fragment. One of the nucleotide substitutions influences the presence of a site for the Hind III restriction endonuclease. That this restriction site indeed discriminates the two non-allelic genes encoding the mouse C4 and C4-Slp isoforms has been demonstrated by Southern blot analysis and nucleotide sequencing at the genomic level. Circumstantial evidence supports the identification of the gene lacking the Hind III site in the region corresponding to the carboxy-terminal end of the C4d fragment as the one encoding the C4-Slp isotype.

scholarly journals A complementary DNA oligomer releases a transcription pause complex.

1983 ◽  
Vol 258 (15) ◽  
pp. 9208-9212 ◽  
Author(s):  
R Fisher ◽  
C Yanofsky
Keyword(s):  
Complementary Dna
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