Inhibition of phospholipase A2 activity by fluosol®, an artificial blood substitute

1982 ◽  
Vol 23 (6) ◽  
pp. 919-927 ◽  
Author(s):  
K.M.Mohames Shakir ◽  
Taffy J. Williams
Keyword(s):  
Phospholipase A2 ◽  
Blood Substitute ◽  
Artificial Blood ◽  
Phospholipase A2 Activity

Sinusoidal Cells in Bone Marrow Take Up BSA-Au Conjugates in the Presence of an Artificial Blood Substitute

1985 ◽  
Vol 43 ◽  
pp. 700-701
Author(s):  
J.S. Geoffroy ◽  
R.P. Becker
Keyword(s):  
Bone Marrow ◽  
Los Angeles ◽  
Iliac Artery ◽  
Blood Substitute ◽  
Sprague Dawley ◽  
Coated Pits ◽  
Sinusoidal Cells ◽  
Artificial Blood ◽  
Left Common Iliac Artery

The pattern of BSA-Au uptake in vivo by endothelial cells of the venous sinuses (sinusoidal cells) of rat bone marrow has been described previously. BSA-Au conjugates are taken up exclusively in coated pits and vesicles, enter and pass through an “endosomal” compartment comprised of smooth-membraned tubules and vacuoles and cup-like bodies, and subsequently reside in multivesicular and dense bodies. The process is very rapid, with BSA-Au reaching secondary lysosmes one minute after presentation. (Figure 1)In further investigations of this process an isolated limb perfusion method using an artificial blood substitute, Oxypherol-ET (O-ET; Alpha Therapeutics, Los Angeles, CA) was developed. Under nembutal anesthesia, male Sprague-Dawley rats were laparotomized. The left common iliac artery and vein were ligated and the right iliac artery was cannulated via the aorta with a small vein catheter. Pump tubing, preprimed with oxygenated 0-ET at 37°C, was connected to the cannula.

Study of phospholipase A2 activity in umbilical endothelial cells in toxemia of pregnancy.

1987 ◽  
Vol 18 (6) ◽  
pp. 582-588
Author(s):  
Hiroyuki SEKI ◽  
Toshiharu JIMBO ◽  
Kazuo SATOH
Keyword(s):  
Endothelial Cells ◽  
Phospholipase A2 ◽  
Phospholipase A2 Activity
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