Prostaglandin F2a stimulates progesterone secretion by porcine luteal cells in vitro throughout the estrous cycle

Oxytocin (OT) is involved in the regulation of steroid secretion by the corpus luteum (CL) in pigs, but OT signal transduction in the porcine CL has not been identified. In this study, the effects of OT on in vitro progesterone (P 4 ) secretion, phosphoinositide (PI) hydrolysis and intracellular mobilisation of Ca 2+ ([Ca 2+ ] i ) were investigated in porcine luteal cells during the early (days 3–5), mid-(days 8–10) and late luteal phases (days 12–14) of the oestrous cycle. Basal concentrations of P 4 and accumulation of inositol phosphates (IPs) were higher (P < 0.05) on days 3–5 and 8–10 of the oestrous cycle than on days 12–14. Basal [Ca 2+ ] i mobilisation did not differ among studied periods of the oestrous cycle. Oxytocin (10 −7 M) enhanced P4 secretion and PI hydrolysis (P < 0.05) by luteal cells harvested on days 8–10 of the oestrous cycle. Moreover, OT started to increase mobilisation of [Ca 2+ ] i at the 15th (days 3–5 and 8–10) or 30th second (days 12–14) in porcine luteal cells. It was concluded that in pigs OT acts as a regulator of steroidogenesis, stimulating P 4 secretion in mature CL. This OT action may be mediated by changes in PI hydrolysis and [Ca 2+ ] i mobilisation.

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Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro

Reproduction ◽

10.1530/rep-06-0178 ◽

2007 ◽

Vol 133 (3) ◽

pp. 641-651 ◽

Cited By ~ 16

Author(s):

Ewa Borowczyk ◽

Mary Lynn Johnson ◽

Jerzy J Bilski ◽

Magda A Bilska ◽

Dale A Redmer ◽

...

Keyword(s):

Gap Junctions ◽

Mrna Expression ◽

Estrous Cycle ◽

Luteal Cell ◽

Gap Junctional Intercellular Communication ◽

Luteal Cells ◽

Progesterone Secretion ◽

Cx43 Mrna

To evaluate the role of gap junctions in the regulation of progesterone secretion, two experiments were conducted. In Experiment 1, luteal cells obtained on days 5, 10, and 15 were cultured overnight at densities of 50×103, 100×103, 300×103, and 600×103cells/dish in medium containing: (1) no treatment (control), (2) LH, or (3) dbcAMP. In Experiment 2, luteal cells from days 5 and 10 of the estrous cycle were transfected with siRNA, which targeted the connexin (Cx) 43 gene. In Experiment 1, progesterone secretion, Cx43 mRNA expression, and the rates of gap junctional intercellular communication (GJIC), were affected by the day of the estrous cycle, cell density, and treatments (LH or dbcAMP). The changes in progesterone secretion were positively correlated with the changes in Cx43 mRNA expression and the rates of GJIC. Cx43 was detected on the luteal cell borders in every culture, and luteal cells expressed 3β-hydroxysteroid dehydrogenase. In Experiment 2, twoCx43gene-targeted sequences decreased Cx43 mRNA expression and progesterone production by luteal cells. The changes in Cx43 mRNA expression were positively correlated with changes in progesterone concentration in media. Thus, our data demonstrate a relationship between gap junctions and progesterone secretion that was supported by (1) the positive correlations between progesterone secretion and Cx43 mRNA expression and GJIC of luteal cells and (2) the inhibition of Cx43 mRNA expression by siRNA that resulted in decreased production of progesterone by luteal cells. This suggests that gap junctions may be involved in the regulation of steroidogenesis in the ovine corpus luteum.

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Polichlorinated biphenyls (PCB126 and PCB 153) action on proliferation and progesterone secretion by cultured in vitro porcine luteal cells

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-2001-18996 ◽

2001 ◽

Vol 109 (08) ◽

pp. 416-418 ◽

Cited By ~ 10

Author(s):

K. Augustowska ◽

A. Wójtowicz ◽

M. Kajta ◽

E. Ropstad ◽

E. Gregoraszczuk

Keyword(s):

Luteal Cells ◽

Progesterone Secretion ◽

Porcine Luteal Cells

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The Effect of Uterine Flushings and Endometrial Protein Fractions on Progesterone Secretion by Porcine Luteal Cells

Acta Veterinaria Brno ◽

10.2754/avb198655030163 ◽

1986 ◽

Vol 55 (3) ◽

pp. 163-171

Author(s):

Jadwiga Przala ◽

Z. Luberda ◽

A. Grazul ◽

T. Wiesak ◽

J. Kotwica

Keyword(s):

Protein Fractions ◽

Luteal Cells ◽

Progesterone Secretion ◽

Porcine Luteal Cells

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Effects of Oxytocin on in Vitro Steroid Release of Midstage Small and Large Porcine Luteal Cells*

Endocrinology ◽

10.1210/endo-126-5-2343 ◽

1990 ◽

Vol 126 (5) ◽

pp. 2343-2349 ◽

Cited By ~ 13

Author(s):

LUTZ PITZEL ◽

HUBERTUS JARRY ◽

WOLFGANG WUTTKE

Keyword(s):

Luteal Cells ◽

Porcine Luteal Cells

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Does prostacyclin (PGI2) support porcine corpus luteum function? – data from in vitro study

Problems of Endocrinology ◽

10.14341/probl201662549 ◽

2016 ◽

Vol 62 (5) ◽

pp. 49

Author(s):

Magdalena Julia Szymańska ◽

Agnieszka Blitek

Keyword(s):

Endothelial Cells ◽

Mrna Expression ◽

Corpus Luteum ◽

Estrous Cycle ◽

In Vitro Study ◽

Luteal Cells ◽

Enzymatic Isolation ◽

Effective Doses ◽

And Function

Background. Prostacyclin (PGI2) of luteal origin is involved in the control of corpus luteum (CL) development and function in cattle. PGI2 may regulate the process of angiogenesis and may stimulate progesterone (P4) secretion by luteal cells via its specific receptors, PTGIR. In contrast to cattle, the role of PGI2 in the pig CL has not yet been described.Aim. The present study aimed to investigate the effect of PGI2 on 1) P4 secretion by luteal cells, and 2) the expression of angiogenesis-related genes in endothelial cells of the porcine CL.Methods. CL collected from gilts on day 5-7 of the estrous cycle were used for enzymatic isolation of luteal (Experiment 1) and endothelial (Experiment 2) cells. In Exp. 1, cultured luteal cells were incubated with increasing (0, 0.01, 0.1, 1, 5 µM) doses of PGI2 analogues: iloprost (ILO) and carbaprostacyclin (cPGI2) for 8 h. To determine the effective doses of PGI2 analogues, P4 concentration in culture medium was examined by RIA. Thereafter, luteal cells were treated with ILO and cPGI2 at the concentration of 1 and 5 µM in the presence or absence of PTGIR antagonist (CAY10441). After 8 h of incubation the medium was collected for P4 determination. In Exp. 2, isolated endothelial cells were treated for 24 h with ILO and cPGI2 at doses of 1 and 5 µM. Then, cells were collected for analysis of Ang-1 and -2 mRNA expression using qPCR.Results. Both, ILO and cPGI2 affected P4 secretion by luteal cells. Elevated levels of P4 were observed in medium after treatment of luteal cells with 1 µM of ILO and 0.1, 1 and 5 µM of cPGI2 compared with control values (p<0.05). The addition of CAY10441 inhibited the stimulatory effect of ILO on P4 secretion, while did not change P4 production by luteal cells incubated with cPGI2. Moreover, PGI2 analogues differentially affected (p<0.05) the expression of proangiogenic factors. ILO stimulated Ang-2, whereas cPGI2 positively affected Ang-1 mRNA expression in endothelial cells at concentrations of 1 µM and 5 µM, respectively.Conclusion. PGI2 affects P4 secretion during luteal phase of the estrous cycle and may regulate the process of angiogenesis in the porcine CL.

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