The role of nutrients, peptide growth factors and co-culture cells in development of preimplantation embryos in vitro

1992 ◽  
Vol 38 (2) ◽  
pp. 297-313 ◽  
Author(s):  
M.T. Kane ◽  
E.W. Carney ◽  
J.E. Ellington
Keyword(s):  
Growth Factors ◽  
Preimplantation Embryos ◽  
Culture Cells ◽  
Peptide Growth Factors

scholarly journals Antiapoptotic Role of Growth Factors in the Myelodysplastic Syndromes: Concordance Between In vitro and In vivo Observations

2005 ◽  
Vol 11 (17) ◽  
pp. 6291-6299 ◽  
Author(s):  
Ramin Tehranchi ◽  
Bengt Fadeel ◽  
Jan Schmidt-Mende ◽  
Ann-Mari Forsblom ◽  
Emma Emanuelsson ◽  
...  
Keyword(s):  
Growth Factors ◽  
Myelodysplastic Syndromes

scholarly journals Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization

Sarcoma ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Katia Bifulco ◽  
Immacolata Longanesi-Cattani ◽  
Maria Teresa Masucci ◽  
Annarosaria De Chiara ◽  
Flavio Fazioli ◽  
...  
Keyword(s):  
Primary Cell Culture ◽  
Lung Metastases ◽  
Urokinase Receptor ◽  
Cell Surfaces ◽  
Chondrosarcoma Cell ◽  
Culture Cells ◽  
Cell Mobilization ◽  
Very High

High levels of urokinase receptor (uPAR) in tissue and serum of patients with chondrosarcoma correlate with poor prognosis. First, we analyzed the uPAR levels in tissues and plasma of five patients affected by chondrosarcoma. Interestingly, very high levels of uPAR and its soluble forms (SuPAR) were found on tumor cell surfaces and plasma, respectively, of two patients with lung metastases. Therefore, to investigate the role of SuPAR in chondrosaromas, we generated a primary cell culture from a chondrosarcoma tissue overexpressing uPAR on cell surfaces. We found that chondrosarcoma-like primary culture cells release a large amount of SuPAR in the medium.In vitro, SuPAR elicits chondrosarcoma cell migration likely through its uPAR88-92sequence, since the DII88-183or DIIDIIR88-284uPAR domains retain motogen effect whereas DI1-87or DIII184-284domains, both lacking the uPAR88-92sequence, are ineffective. Chondrosarcoma cells cross matrigel in response to SuPAR, and their invasion capability is abrogated by RERF peptide which inhibits uPAR88-92signalling. These findings assign a role to uPAR in mobilizing chondrosarcoma cells and suggest that RERF peptide may be regarded as a prototype to generate new therapeutics for the chondrosarcoma treatment.

scholarly journals Transcription of paternal Y-linked genes in the human zygote as early as the pronucleate stage

Zygote ◽  
1994 ◽  
Vol 2 (4) ◽  
pp. 281-287 ◽  
Author(s):  
Asangla Ao ◽  
Robert P. Erickson ◽  
Robert M.L. Winston ◽  
Alan H Handysude
Keyword(s):  
Mammalian Species ◽  
Blastocyst Stage ◽  
Preimplantation Embryos ◽  
Human Embryos ◽  
Cell Stage ◽  
Gonad Differentiation ◽  
Embryonic Genome ◽  
Human Preimplantation Embryos

SummaryGlobal activation of the embryonic genome occurs at the 4– to 8–cell stage in human embryos and is marked by continuation of early cleavage divisions in the presence of transcriptional inhibitors. Here we demonstrate, using recerse transcripase–polymerase chin reaction (Rt–PCR), the presence of transcripts for wo paternal Y chromosomal genes, ZFY and SRY in human preimplantation embryos. ZFY transcripts were detected as early as the pronucleate stage, 20–24 h post-insemination In vitro and at intermediate stages up to the blastocyst stage. SRY Transcripts were also detected at 2–cell to blastocyos observed in many mammalian species focuses attention on the role of events in six determination prior to gonad differentiation.

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