Effects of differentiating agents on a trophoblast cell, line as revealed by in vitro studies

The primary sheep trophoblast cells (STCs) have a finite lifespan in culture. This feature limits the scope for long-termin vitrostudies with STCs. This study was an attempt to establish and characterize a telomerase-immortalized sheep trophoblast cell line. STCs were isolated and purified by using Percoll and specific immunoaffinity purification, respectively. The purified STCs were transfected with a plasmid carrying sequences of human telomerase reverse transcriptase (hTERT) to create immortalized sheep trophoblast cell line (hTERT-STCs). hTERT-STCs showed a stable expression of hTERT gene, serially passaged for a year, and showed active proliferation without signs of senescence. Cytokeratin 7 (CK-7), secreted human chorionic gonadotrophin subunitβ(CG-β), placental lactogen (PL), and endogenous jaagsiekte sheep retrovirus (enJSRV) envelope genes were expressed in hTERT-STCs. Transwell cell invasion assay indicated that hTERT-STCs still possessed the same invasive characteristics as normal primary sheep trophoblast cells. hTERT-STCs could not grow in soft agar and did not develop into tumors in nude mice. In this study, we established a strain of immortalized sheep trophoblast cell line which could be gainfully employed in the future as an experimental model to study trophoblast cells with secretory function, invasive features, and probable biological function of enJSRV envelope genes.

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Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro

Archives of Biological Sciences ◽

10.2298/abs1304331c ◽

2013 ◽

Vol 65 (4) ◽

pp. 1331-1337 ◽

Cited By ~ 1

Author(s):

Danica Cujic ◽

Zanka Bojic-Trbojevic ◽

Natasa Tosic ◽

Sonja Pavlovic ◽

Ljiljana Vicovac

Keyword(s):

Cell Line ◽

Culture Media ◽

Mrna Level ◽

Trophoblast Cell ◽

Mrna Levels ◽

Human Trophoblast ◽

Present Evidence ◽

Trophoblast Cell Line ◽

Synthetic Glucocorticoid

Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined.

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The effect of IL-6 on the trophoblast cell line HTR-8/SVneo

Archives of Biological Sciences ◽

10.2298/abs1003531j ◽

2010 ◽

Vol 62 (3) ◽

pp. 531-538 ◽

Cited By ~ 3

Author(s):

Milica Jovanovic ◽

Tamara Kovacevic ◽

Ivana Stefanoska ◽

Ljiljana Vicovac

Keyword(s):

Cell Line ◽

Normal Pregnancy ◽

Blastocyst Stage ◽

Uterine Wall ◽

Trophoblast Cell ◽

Trophoblast Invasion ◽

Invasion And Migration ◽

And Migration ◽

Trophoblast Cell Line

Embryonic development up to the blastocyst stage, implantation into the uterine wall and the development of the functional placenta are steps crucial for the establishment of normal pregnancy. Specific cells of the placenta, the trophoblast cells, invade the uterine stroma and spiral arteries, adapting them to pregnancy. Interleukin-6 is present in the human endometrium during the receptive phase and early pregnancy. Trophoblasts also produce IL-6, which was found to stimulate trophoblast invasion and migration in vitro. Here we show that the activity of MMP-9 may contribute to the observed increased invasion. In addition, in the HTR-8/SVneo trophoblast cell line IL-6 increases cell proliferation. .

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Effects of hypoxia and hyperglycemia on proliferation and expression of glucose-related signaling molecules in extravillous trophoblast cell line in vitro

10.5353/th_b4129079 ◽

2008 ◽

Author(s):

Yuk-ling Chan

Keyword(s):

Cell Line ◽

Signaling Molecules ◽

Trophoblast Cell ◽

Extravillous Trophoblast ◽

Trophoblast Cell Line

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Arterial endothelial cytokines guide extravillous trophoblast invasion towards spiral arteries; an in-vitro study with the trophoblast cell line ACH-3P and female non-uterine endothelial cells

Placenta ◽

10.1016/j.placenta.2015.12.010 ◽

2016 ◽

Vol 38 ◽

pp. 49-56 ◽

Cited By ~ 4

Author(s):

Gregor Weiss ◽

Berthold Huppertz ◽

Monika Siwetz ◽

Ingrid Lang ◽

Gerit Moser

Keyword(s):

Endothelial Cells ◽

Cell Line ◽

In Vitro Study ◽

Trophoblast Cell ◽

Vitro Study ◽

Extravillous Trophoblast ◽

Trophoblast Invasion ◽

Trophoblast Cell Line

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Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71

FEBS Open Bio ◽

10.1002/2211-5463.13087 ◽

2021 ◽

Author(s):

Da Som Jeong ◽

Myoung Hee Kim ◽

Ji‐Yeon Lee

Keyword(s):

Gene Expression ◽

Cell Line ◽

Trophoblast Cell ◽

Human Trophoblast ◽

Trophoblast Cell Line

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MAML1: a coregulator that alters endometrial epithelial cell adhesive capacity

Fertility Research and Practice ◽

10.1186/s40738-021-00100-y ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Sadaf Zafir ◽

Wei Zhou ◽

Ellen Menkhorst ◽

Leilani Santos ◽

Evdokia Dimitriadis

Keyword(s):

Notch Signaling ◽

Cell Line ◽

Notch Signaling Pathway ◽

Endometrial Receptivity ◽

Trophoblast Cell ◽

Luminal Epithelium ◽

Secretory Phase ◽

Ishikawa Cells ◽

Trophoblast Cell Line ◽

Adhesive Capacity

Abstract Background Abnormalities in endometrial receptivity has been identified as a major barrier to successful embryo implantation. Endometrial receptivity refers to the conformational and biochemical changes occurring in the endometrial epithelial layer which make it adhesive and receptive to blastocyst attachment. This takes place during the mid-secretory phase of woman’s menstrual cycle and is a result of a delicate interplay between numerous hormones, cytokines and other factors. Outside of this window, the endometrium is refractory to an implanting blastocyst. It has been shown that Notch ligands and receptors are dysregulated in the endometrium of infertile women. Mastermind Like Transcriptional Coactivator 1 (MAML1) is a known coactivator of the Notch signaling pathway. This study aimed to determine the role of MAML1 in regulating endometrial receptivity. Methods The expression and localization of MAML1 in the fertile human endometrium (non-receptive proliferative phase versus receptive mid-secretory phase) were determined by immunohistochemistry. Ishikawa cells were used as an endometrial epithelial model to investigate the functional consequences of MAML1 knockdown on endometrial adhesive capacity to HTR8/SVneo (trophoblast cell line) spheroids. After MAML1 knockdown in Ishikawa cells, the expression of endometrial receptivity markers and Notch dependent and independent pathway members were assessed by qPCR. Two-tailed unpaired or paired student’s t-test were used for statistical analysis with a significance threshold of P < 0.05. Results MAML1 was localized in the luminal epithelium, glandular epithelium and stroma of human endometrium and the increased expression identified in the mid-secretory phase was restricted only to the luminal epithelium (P < 0.05). Functional analysis using Ishikawa cells demonstrated that knockdown of MAML1 significantly reduced epithelial adhesive capacity (P < 0.01) to HTR8/SVneo (trophoblast cell line) spheroids compared to control. MAML1 knockdown significantly affected the expression of classical receptivity markers (SPP1, DPP4) and this response was not directly via hormone receptors. The expression level of Hippo pathway target Ankyrin repeat domain-containing protein 1 (ANKRD1) was also affected after MAML1 knockdown in Ishikawa cells. Conclusion Our data strongly suggest that MAML1 is involved in regulating the endometrial adhesive capacity and may facilitate embryo attachment, either directly or indirectly through the Notch signaling pathway.

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